Pyruvate kinase M2 activators promote tetramer formation and …
Following lysis and immunoprecipitation with Flag antibodies, we determined the relative amount of endogenous PKM2 that co-precipitated under the various conditions by western blot using …
KITENIN promotes aerobic glycolysis through PKM2 induction by ...
2023年8月8日 · To determine whether si-PKM2 specifically reduces PKM2 expression, we performed Western blotting to measure the protein expression of PKM2, PKM1, and KITENIN …
Pyruvate kinase M2 (PKM2) interacts with activating transcription ...
2021年12月1日 · Western blot analysis indicated that knocking down PKM2 by LV-sh-PKM2 significantly reduced the expression of inflammatory factors iNOS, pyroptosis-related factors …
Pyruvate kinase M2 activation reprograms mitochondria in CD8 T …
2025年4月7日 · PKM2 protein tetramer level was detected by western blot. Isolated CD8 T cells from spleen of pMel-1 mice (1.5 ×10 6 cells per ml) were activated by gp100 (1 μM) in the …
The glycolytic enzyme PKM2 regulates inflammatory …
Using murine bone marrow-derived macrophages (BMMs) stimulated with lipopolysaccharides (LPS) to mimic inflammatory conditions in vitro, we analyzed PKM2 expression and glycolytic …
PKM2 crotonylation reprograms glycolysis in VSMCs ... - Nature
2025年4月3日 · In PDGF-BB-induced synthetic VSMCs, PKM2 crotonylation was upregulated and promotes its nuclear translocation, thereby facilitating the expression of Glut1 and Ldha. …
PKM2蛋白在细胞代谢中的活性检测—艾普蒂生物 - 哔哩哔哩
2024年9月10日 · Western Blot验证：通过Western Blot分析PKM2的表达与磷酸化状态，确认活性变 化是否与PKM2的激活或失活相关。 代谢活性分析：通过乳酸生成和葡萄糖摄取检测，进 …
PKM2–c-Myc–Survivin Cascade Regulates the Cell Proliferation, …
2020年9月8日 · Western blotting was used to analyze PKM2 and c-Myc proteins in MCF-7 and MDA-MB-231 cells. β -Actin was used as a loading control. (E) 293T cells were transfected …
PKM2 is a key factor to regulate neurogenesis and cognition by ...
2025年1月14日 · Western blot analysis confirmed significant reductions in MCT2 and HCAR1 expression in cultured NSPCs in vitro, indicating effective knockdown efficiency (Figures S4A …
The glycolytic enzyme PKM2 regulates inflammatory …
2025年4月1日 · Western blot analysis of PKM2 expression in the nucleus and cytoplasm of control and inflammation-stimulated groups revealed a significant increase in nuclear PKM2 in …