PKM2 promotes pulmonary fibrosis by stabilizing TGF-β1
2022年9月21日 · To determine the role of PKM2 in pulmonary fibrosis, we first analyzed PKM2 mRNA expression in a published Gene Expression Omnibus (GEO) dataset of IPF lungs (GSE24206) and found that PKM2 mRNA level was significantly increased with IPF …
Pyruvate kinase M2 activators promote tetramer formation and
2012年8月26日 · Coprecipitating endogenous PKM2 in lysates from A549 cells stably expressing Flag-PKM2 (WT) or Flag-PKM2 K305Q as assessed by western blotting using an antibody that recognizes PKM2 or...
CIP2A induces PKM2 tetramer formation and oxidative ... - Nature
2024年2月6日 · Flag-CIP2A and shRNA-resistant HA-tagged PKM2 (WT or S287A mutant) were transfected into PKM2-depleted H1299 cells, followed by IP and western blotting analysis with the indicated...
Role of pyruvate kinase M2-mediated metabolic reprogramming …
2020年5月11日 · Western blotting revealed that PKM2 was almost totally eliminated in Pkm2 −/−-podocytes (Fig. 7a, b), and the trace amount of PKM2 appeared probably because of the purity (≥95%) of the ...
KITENIN promotes aerobic glycolysis through PKM2 induction by ...
2023年8月8日 · To determine whether si-PKM2 specifically reduces PKM2 expression, we performed Western blotting to measure the protein expression of PKM2, PKM1, and KITENIN in CaCo2/EV and CaCo2/KITENIN cells (Additional file 1: Fig S8A, B); and qRT-PCR to measure the mRNA expression of KITENIN, ErbB4, and the mediators of …
Pyruvate Kinase M2 Tetramerization Protects against Hepatic Stellate ...
The PKM2 protein was detected by immunofluorescence and Western blot at an early stage of HSC activation, confirming that PKM2 expression increased in parallel with that of the HSC activation and fibrosis markers [α-SMA, collagen 1, fibronectin, and platelet-derived growth factor receptor-β (PDGFRβ, alias PDGFRB)] (Figure 3 A).
Pyruvate Kinase M2 Promotes the Activation of Dendritic Cells by ...
2020年5月26日 · Using the Click-iT O-GlcNAc enzymatic labeling system, O-GlcNAcylated proteins were biotinylated, precipitated, and analyzed for PKM2 using western blotting. Samples prepared with MCF-7 cells were loaded as a positive control.
Article Pyruvate Kinase M2 Regulates Hif-1α Activity and IL-1β ...
2015年1月6日 · Fractions collected were analyzed by western blotting detecting PKM2 and the result reveals a shift in the molecular weight of PKM2 with the appearance of a band corresponding to approximately 250 kDa (Figure 1 G, elution volume 10–11 ml) and a reduction of the smaller molecular weight complexes (elution volume 13–14), again suggesting a ...
Positive feedback regulation of microglial glucose metabolism by ...
2022年4月5日 · We next used western blotting to measure PKM2 levels in isolated adult microglia and detected remarkably increased microglial accumulation of PKM2 in 6-month-old 5XFAD mice compared with the age-matched WT mice (Figure 3 A).
PKM2‐Driven Lactate Overproduction Triggers …
2024年10月30日 · Western blotting validated the knockdown efficiency of the PKM2 specific siRNAs (Figure 2A). The results indicated that the expression of endothelial marker CD31, was notably decreased after hypoxic challenge, whereas that of the mesenchymal marker, α-SMA, was upregulated (Figure 2B ).