2007年6月1日 · Among the molecules with important biological function induced by IFN is the doubled-stranded (ds) RNA-dependent protein kinase (PKR), an enzyme with multiple effects in cells, which plays a critical role in the antiviral defence mechanism of the host [5].

Here, we show that dsRNA induces the formation of a novel condensate by PKR that is distinct from other known ribonucleoprotein assemblies. These results challenge prior observations that PKR is recruited to stress granules and suggest that the condensation of PKR may be a mechanism that cells use to modulate PKR activation.

2008年8月29日 · Upon binding double-stranded RNA (dsRNA), PKR undergoes autophosphorylation reactions that activate the kinase. PKR then phosphorylates eukaryotic initiation factor 2alpha, thus inhibiting protein synthesis in virally infected cells. Using a series of dsRNAs of increasing length, we define the mechanism of PKR activation.

Upon binding dsRNA, PKR undergoes autophosphorylation reactions that activate the kinase. PKR then phosphorylates eIF2α, thus inhibiting protein synthesis in virally-infected cells. Here, we define the mechanism of PKR activation using a series dsRNAs of increasing length.

The IFN-induced double-stranded RNA-dependent protein kinase (PKR) is one of the four mammalian serine-threonine kinases (the three others being HRI, GCN2 and PERK) that phosphorylate the eIF2 alpha translation initiation factor, in response to stress signals, mainly as a result of viral infections. …

2005年4月22日 · A key player in these dsRNA-mediated events is PKR (protein kinase RNA-dependent), an IFN-inducible serine/threonine protein kinase that autophosphorylates following association with...

2024年8月27日 · Wild-type or editing-inactive human ADAR1 expressed in A549 cells inhibits activation of endogenous PKR. ADAR1 dsRNA binding is required for, but is not sufficient for, PKR inhibition. Mutating the ADAR1 dsRBD3-PKR contact prevents co-immunoprecipitation, ADAR1 inhibition of PKR activity, and co-localization of ADAR1 and PKR in cells.

2017年6月13日 · We propose that bivalent interactions with the double stranded RNA binding domain and the basic region underlie the ability of RNAs containing limited structure to activate PKR by enhancing...

The dsRNA-activated protein kinase PKR participates in several cellular signaling pathways in addition to controlling protein translation through eIF2α phosphorylation. PKR was initially thought to be an essential actor in the mechanism of action of IFN, given the number of viral proteins that were reported to counteract its action.

2022年8月15日 · However, intracellular long dsRNA was sensed by PKR and TLR3, which activate distinct signals, and synergistically induced rapid apoptosis. PKR essentially induced translational arrest,...