Saccharomyces cerevisiae Abz2 is a pyridoxal 5′-phosphate (PLP)-dependent lyase that converts 4-amino-4-deoxychorismate (ADC) to para-aminobenzoate and pyruvate. To investigate the catalytic mechanism, we determined the 1.9 Å resolution crystal structure of Abz2 complexed with PLP, representing the first eukaryotic ADC lyase structure.

酿酒酵母 Abz2 是一种 5'-磷酸吡哆醛 (PLP) 依赖性裂解酶，可将 4-氨基-4-脱氧分支酸 (ADC) 转化为对氨基苯甲酸和丙酮酸。 为了研究催化机制，我们确定了与 PLP 复合的 Abz2 的 1.9 A 分辨率晶体结构，代表了第一个真核 ADC 裂解酶结构。

【摘 要】β-丙氨酸作为一种重要的平台化合物,是合成含氮化合物的前体.相较于化学合成法,人们更倾向于利用l-天冬氨酸-α 脱羧酶(adc)环保地制备 β-丙氨酸.由于细菌来源的adc存在严重的机理性底物失活,限制了其在工业上的应用.实验在大肠杆菌中表达了3种古 ...

2013年7月1日 · Saccharomyces cerevisiae Abz2 is a pyridoxal 5′-phosphate (PLP)-dependent lyase that converts 4-amino-4-deoxychorismate (ADC) to para-aminobenzoate and pyruvate....

Pyridoxal 5′-phosphate (PLP)-dependent basic amino acid decarboxylases from the β/α-barrel-fold class (group IV) exist in most organisms and catalyze the decarboxylation of diverse substrates, essential for polyamine and lysine biosynthesis.

Aminodeoxychorismate lyase (ADCL) is a kind of pyridoxal-5'-phosphate (PLP)-dependent enzyme that catalyzes the conversion of 4-amino-4-deoxychorismate (ADC) to...

2020年10月2日 · ADC is homologous to eukaryotic ornithine decarboxylase (ODC) and diaminopimelate decarboxylase (DapDC), which are fold type III PLP-dependent enzymes that contain an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain, similar to bacterial alanine racemases.

2013年8月9日 · Saccharomyces cerevisiae Abz2 is a pyridoxal 5'-phosphate (PLP)-dependent lyase that converts 4-amino-4-deoxychorismate (ADC) to para-aminobenzoate and pyruvate. To investigate the catalytic mechanism, we determined the 1.9 Å resolution crystal structure of Abz2 complexed with PLP, representing the …

2000年7月1日 · 4-氨基-4-脱氧胆酸酯裂解酶（ADCL）是PLP依赖性酶的折叠类型IV的成员，该酶将4-氨基-4-脱氧胆酸酯（ADC）转换为对氨基苯甲酸酯和丙酮酸。 使用MIR相结合密度修饰已解决了来自大肠杆菌的ADCL的晶体结构。 该结构在2.2 A分辨率下已精炼到20.6％的R系数。 该酶是具有结晶学双重轴的同型二聚体，并且多肽链通过域间环折叠成小域和大域。 辅酶5'-磷酸吡ido醛位于结构域界面，其背面朝向蛋白质。 尽管活性位点的主链折叠与D-氨基酸和L-支链氨基酸氨 …

2013年8月9日 · Saccharomyces cerevisiae Abz2 is a pyridoxal 5′-phosphate (PLP)-dependent lyase that converts 4-amino-4-deoxychorismate (ADC) to para-aminobenzoate and pyruvate. To investigate the catalytic mechanism, we determined the 1.9 Å resolution crystal structure of Abz2 complexed with PLP, representing the first eukaryotic ADC lyase structure.