2023年11月1日 · Strengthening of intramolecular interactions and expansion of the angle between the substrate-binding pocket and the pyridoxal 5′-phosphate (PLP)-binding pocket …

在近期一项关于荧光假单胞菌Pseudomonas fluorescens来源的S型ω-转氨酶的稳定性改造研究中，Börner等 基于底物诱导酶失活的机理将突变区域限制在辅酶结合区域，对该区域32个位点 …

2018年9月20日 · In this work, the stability of Vf -ATA was explored. First, the affinity between enzyme and pyridoxal-5'-phosphate (PLP) (KD value of 7.9 μM) was determined. Addition of …

This article details the structural features and catalytic mechanisms of typical PLP-dependent enzymes such as ω-transaminase, lysine decarboxylase, threonine aldolase, and L-tyrosine …

2020年11月1日 · Compared with the asymmetric hydrogenation of enamines catalyzed by a transition metal, asymmetric transamination catalyzed by ω-amine transaminase (ω-ATA) …

2019年11月18日 · Recently published data support the hypothesis of a key role for the cofactor pyridoxal-5′-phosphate (PLP) in the stability of both dimeric and tetrameric (S)-selective …

2020年11月26日 · Amine transaminases (ATAs) are used to synthesize enantiomerically pure amines, which are building blocks for pharmaceuticals and agrochemicals. R -selective ATAs …

通过结构和序列信息分析，进一步发现分子内相互作用的加强以及底物结合口袋和5’-磷酸吡哆醛（plp）结合口袋之间的角度扩大是atata热稳定性和活性同步增强的重要原因。最后，口袋工 …

2022年2月22日 · Recently, two interesting approaches involving imine reductases for the synthesis of chiral piperazines 8 and 1,4-diazepanes 9 have been described (Scheme 1 A). …

2010年6月17日 · α-Transaminases are pyridoxal 5′-phosphate (PLP)-dependent enzymes widespread in nature for the synthesis of α-amino acids from the corresponding α-keto acids; …