HP1-mediated silencing targets Pol II coactivator complexes
2008年2月10日 · We analyzed HP1-mediated inhibition of preinitiation complex (PIC) assembly in vitro on chromatin templates regulated by GAL4-VP16 or Sp1. HP1 blocked key subunits of the TFIID and Mediator...
The ability of HP1 to interact with the Hsp70 RNA in vivo was confirmed by primer extension on the population of RNA immunoprecipitated from SL-2 cells with the C1A9 (anti-HP1) antibody...
Little is known of the specific biochemical mechanism by which heterochromatin protein 1 (HP1) inactivates a gene. We analyzed HP1-mediated inhibition of preinitiation complex (PIC) assembly in...
HP1 maintains protein stability of H3K9 methyltransferases and ...
2022年2月15日 · Heterochromatin protein 1 (HP1) specifically recognizes H3K9me2/3, contributing to transcriptional suppression and spread of H3K9me2/3. Here, we demonstrate another role of HP1 in heterochromatin organization: regulation of protein stability of H3K9 methyltransferases (H3K9 MTs) and demethylases (H3K9 DMs).
HP1 proteins regulate nucleolar structure and function by …
2022年12月19日 · Hp1α/β deficiency reduces cell proliferation, ribosomal RNA biosynthesis and mobility of Nucleophosmin, a major nucleolar component. Nucleolar integrity and function require HP1α/β proteins to be recruited to H3K9me3-marked PCH and their ability to dimerize.
The alternative role of DNA methylation in splicing regulation
The first involves modulation of the elongation rate of RNA polymerase II (Pol II) by CCCTC-binding factor (CTCF) and methyl-CpG binding protein 2 (MeCP2); the second involves the formation of a protein bridge by heterochromatin protein 1 (HP1) that recruits splicing factors onto transcribed alternative exons.
Functions of HP1 proteins in transcriptional regulation - PMC
Non-histone chromatin proteins such as the Heterochromatin Protein 1 (HP1) proteins are critical regulators of transcription, contributing to gene regulation through a variety of molecular mechanisms. HP1 proteins are highly conserved, and many eukaryotic genomes contain multiple HP1 …
生命科学学院季雄团队揭示RNA聚合酶II亚基异质性和CTCF绝缘机理
2022年9月15日，北京大学生命科学学院季雄研究员课题组在Molecular Cell杂志在线发表了题为“ Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity ”的研究论文。...
H3K9 methylation-independent activity for HPL-2/HP1 in ... - bioRxiv
2024年9月29日 · We show that HPL-2/HP1 deficient in methyl-lysine binding or in the absence of H3K9me can still repress transcription, promote organogenesis and localize to heterochromatin foci.
生命科学学院季雄团队揭示RNA聚合酶亚基RPB7偶联磷酸酶CTDP1稳定Pol …
5 天之前 · 这些结果一致表明RPB7与新生RNA加工过程密切相关。 综上所述，本研究鉴定到Pol II亚基RPB7的关键互作蛋白——磷酸酶CTDP1，并揭示了RPB7与CTDP1协同调控Pol II蛋白稳定性及其转录再起始过程的分子机制。