2007年3月15日 · Simultaneously, DNMTs target negative regulators of memory, such as PP1, for transcriptional silencing. DNMT activation results in increased methylation of the PP1 gene, which can lead to the recruitment of MBDs to this locus.

DNMT inhibition prevents the PP1 methylation increase, resulting in aberrant transcription of the gene during the memory-consolidation period. These results demonstrate that DNA methylation is dynamically regulated in the adult nervous system and that this cellular mecha-nism is a crucial step in memory formation.

In this study, we compared the phenotypes of conditional mutant mice deficient for either Dnmt1 or Dnmt3a (or both) exclusively in the forebrain postnatal postmitotic neurons. We aim to address whether Dnmt1 and Dnmt3a play a redundant role in the maintenance of DNA methylation in postmitotic neurons.

2010年3月14日 · We generated conditional mutant mice that lack Dnmt1, Dnmt3a or both exclusively in forebrain excitatory neurons and found that only double knockout (DKO) mice showed abnormal long-term plasticity...

2020年4月1日 · Another study detected increased levels of methylated PP1 along with mRNA downregulation in the hippocampus after contextual fear training (Miller and Sweatt, 2007). These findings indicate that dynamic DNA methylation may represent a means to relieve the memory constraint upon stimulation through the regulation of gene expressions.

2017年5月17日 · DNMT3A and 3B are the main de novo DNA methyltransferases (DNMTs) in the brain that introduce new methylation marks to non-methylated DNA in postmitotic neurons. DNA methylation is a key epigenetic mark that is known to regulate important cellular processes in neuronal development and brain plasticity.

Protein phosphatase 1 (PP1)γ, which critically regulates neuroprotective pathways in brain, was measured in mRNA and protein activity and promoter methylation. DNMT1 was unchanged in H1 and H4, while DNMT3A and DNMT3B were decreased in H4. The mRNA and protein levels of PP1γ were decreased in H4.

2007年3月15日 · DNMT inhibition prevents the PP1 methylation increase, resulting in aberrant transcription of the gene during the memory-consolidation period. These results demonstrate that DNA methylation is dynamically regulated in the adult nervous system and that this cellular mechanism is a crucial step in memory formation.

Using a gene microarray analysis, we demonstrated that the DNMT1 transgene had a profound influence on the inflammatory pathways and the PPAR signaling pathway in the macrophages in response to LPS...

2007年3月15日 · DNMT inhibition prevents the PP1 methylation increase, resulting in aberrant transcription of the gene during the memory-consolidation period. These results demonstrate that DNA methylation is dynamically regulated in the adult nervous system and that this cellular mechanism is a crucial step in memory formation.