2013年3月5日 · VHL-deficient PRC3 cells were more sensitive to treatment with 968, compared to the VHL-reconstituted WT8 cells (Figure 7A). To confirm that this is not only a cell-line-specific phenomenon, we also cultured UMRC2 cells in the presence of 968 or diluent control and showed selective sensitivity of VHL -deficient cells ( Figure 7 B).

Microvasculature with abundant endothelial fenestration (EFs) on VHL null clear cell renal cell carcinoma (ccRCC) might be vascular endothelial growth factor (VEGF)‐dependent capillaries and sensitive to anti‐VEGF therapy. (A) The graph shows a significant reduction of EFs in VHL −/− pRC3 xenografts by the bevacizumab treatment. (B ...

2011年10月24日 · Short-hairpin RNA (shRNA)-mediated knockdown of JunB in 786-O and A498 VHL null ccRCC cells suppressed their invasiveness. In addition, JunB knockdown significantly repressed tumor growth and...

2004年2月1日 · VHL−/− cells constitutively overexpress hypoxia-inducible genes through both transcriptional and posttranscriptional mechanisms and form tumors when injected into nude mice. Reintroduction of pVHL into VHL−/− cell lines restores normal oxygen-dependent regulation of these genes and suppresses tumor formation in the mouse xenograft assay.

2005年6月14日 · In the paired cell lines, PRC3 and WT8, EGFR protein was elevated post-transcriptionally in the VHL mutant and EGF-stimulated phosphorylation was prolonged. We propose that combined EGFR and...

The VHL tumor suppressor protein (pVHL) is part of an E3 ubiquitin ligase that targets HIF for destruction. pVHL-defective renal carcinoma cells exhibit increased NF-kB activity but the mechanism is unclear. NF-kB affects tumori-genesis and therapeutic resistance in some settings.

786-O cells express HIF-2 ␣ but not HIF-1 ␣ . 786-O (PRC-3) is a VHL -defective renal carcinoma cell line, whereas 786-O (WT-8) cells have been stably transfected with functional VHL (38)....

2013年3月5日 · Herein, we performed MFA using a combined model of [U-13 C 6]glucose and [1-13 C 1]glutamine tracer data sets from the 786-O derived isogenic clones PRC3 (VHL −/−)/WT8 (VHL +) cells, which show a robust metabolic regulation by reintroduction of pVHL. To this end, we first determined specific glucose/glutamine consumption and lactate ...

2003年12月5日 · Cell Culture and Reagents—The pVHL-deficient 786-O cell clones stably expressing wild type VHL (WT10), truncated VHL (1–115) or empty plasmid (PRC3) were kindly provided by Dr. Kaelin (Dana-Farber Institute, Boston, MA). Parental VHL-negative RCC4 cells (herein designated RCC4–) and the corresponding VHL-stable transfectants (RCC4+) were ...

2007年12月13日 · Loss of the tumor suppressor gene von Hippel–Lindau (VHL) plays a key role in the oncogenesis of clear cell renal cell carcinoma (CCRCC). The loss leads to stabilization of the HIF transcription complex, which induces angiogenic and mitogenic pathways essential for tumor formation.