DNA-based short antisense oligonucleotides (AS-ON) can effectively target PRRSV NSP9, thus inhibiting PRRSV replication in MARC-145 cells and PAMs (52). Two types of AS-ONs, modified by locked...

2024年2月20日 · Following a 5-day incubation at 37°C in a CO 2-controlled environment, the cytopathic effect (CPE) induced by PRRSV was meticulously evaluated using an inverted microscope (Olympus Corporation, Tokyo, Japan). The CPE was characterized by notable changes in Pams-163 cells, including cell shrinkage, detachment, and the blurring of cellular ...

2006年11月2日 · The results demonstrate two distinct mechanisms of PRRSV infection: primary infection of a relatively small subpopulation of innately PRRSV-permissive cells, and secondary cell-to-cell transmission to contiguous cells which appear non-permissive to free virus. The results also indicate that an intac …

2023年2月11日 · PRRSV在Marc145细胞上生长可产生细胞病变效应（CPE）。 PRRSV感染Marc145细胞过程可大致分为两个阶段：第一阶段，病毒感染细胞后的20～22h，仅部分细胞被随机感染，而且Marc145细胞对PRRSV的低感染性与感染剂量无关，因为感染剂量比常规量提高100倍，感染22h后，仅5.5%的细胞呈PRRSV阳性；第二阶段，感染后的2～3d，也称为病毒的对数感染期，病毒感染蔓延是通过相邻细胞之间的接触感染，细胞对自由病毒不敏感，并出现感 …

2013年11月5日 · respiratorysyndrome,PRRS)是由猪生殖与呼吸系统综合征病毒 (PRRSV)引起的一种新的有高度传染性的猪病,以母猪发热、厌食和流产、木乃伊胎、死产、弱仔等繁殖障碍以及仔猪的呼吸道症状和高死亡率为特征。 PRRS自1987年在美国和. 1990年在欧洲被发现以来,已传遍世界所有养猪国家。 1991年荷兰Wensvort等在Lelystad首次分离到病原,命名为Lelystadvirus,简称LV;1992年美国也分离到病原,命名为. ATCCCR-2332株。 同年被国际兽疫局 (OIE)列为需要 …

2006年11月2日 · Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiologic agent of PRRS, causing widespread chronic infections which are largely uncontrolled by currently available vaccines or other antiviral measures. Cultured monkey kidney (MARC-145) cells provide an important tool for the study of PRRSV replication.

When MARC-145 cells were transfected with shRNA expression vectors and then infected with PRRSV, N179 was found to be the most effective inhibition site in decreasing cytopathic effect (CPE) induced by PRRSV.

2013年10月31日 · Here we report the rescue of a recombinant porcine reproductive and respiratory syndrome virus (PRRSV) carrying an enhanced green fluorescent protein (EGFP) reporter gene as a separate transcription unit.

2020年8月21日 · CPE of the PRRSV strain HN in porcine PMECs. To test the susceptibility of porcine PMECs to PRRSV, cells were infected or mock-infected with the PRRSV strain HN and observed daily for CPE. From five days post-infection (dpi) onwards, increased cell degeneration was observed and some cells shriveled and detached from the wall of the wells.

2019年11月15日 · 结果表明，临床病料RT-PCR检测呈PRRSV核酸阳性，处理后的病料接种至Marc-145细胞72h之后产生CPE，间接免疫荧光鉴定为PRRSV抗原阳性。序列拼接显示分离株全基因组开放阅读框大小为15119bp。