pUC8-SZ - Addgene
Plasmid pUC8-SZ from Dr. Takeshi Sano's lab contains the insert streptavidin and is published in Nucleic Acids Res. 1986 Feb 25. 14 (4):1871-82. This plasmid is available through Addgene.
pUC8质粒图谱、载体图谱、序列、价格、抗性、测序引物、大小 …
pUC8载体价格4800元，pUC8载体质粒图谱 (Vector map)，载体序列 (Sequence)见下文，载体质粒抗性为氨苄，质粒大小为2.7 kb。
pUC Vector: Features, Selectable markers, Blue White Screening …
Advantages of pUC vector. High copy number 500-600 copies per cell; Easy one step selection of recombinant colonies; Many restriction sites in MCS (Multiple Cloning Sites) Disadvantage. Insert size is 15 Kb; Learn More: Insertional-inactivation-in …
Pbr322 and puc8 plasmids | PDF - SlideShare
2015年2月3日 · 2. pUC8 Plasmid The pUC8 plasmid was designed by scientists and contains the lac z gene. To produce the plasmid, the pBR322 plasmid was cut in half with EcoR I and the section containing the ampicillin resistance gene was combined with …
Physical maps of plasmids pUC8 and pUC8:15. pUC8 is a …
Plasmid pUC8:15 bears a 2.3-kb Vitreoscilla chromosomal fragment (Hind IIIHind III) containing the Vitreoscilla hemoglobin gene (vgb). ori, origin of replication of plasmid pMB1; Amp R,...
Understanding the pUC8 Plasmid Vector - Genspark
pUC8 is often used in blue/white screening, where recombinant colonies appear white due to the disruption of the lacZ gene. The plasmid is used in various applications, including the study of gene expression and the effects of drugs on target genes. Origin: Derived from pBR322 and M13mp8, pUC8 includes elements from both.
8 Plasmid Cloning Vectors - ScienceDirect
1988年1月1日 · This chapter discusses the present generation of plasmid cloning vectors. It is not a comprehensive listing but rather a sampling of the most recent plasmids that illustrate the general trends in vector design. This is a massive tome on “cloning vectors” whose loose-leaf binder design allows new descriptions to be fitted into this chapter.
Vector Database - Addgene
Assembled from pUC18 and M13mp8 by F. Pfeiffer, MPI, Martinsried Hosts: E.coli JM83, E.coli JM105, E.coli JM109, E.coli NM522. Related vectors: pUC7, M13mp8, pUC9. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) How was your experience today? Addgene is a nonprofit plasmid repository.
'8' stands for order of discoveryin the same laboratory Construction — It is derived from pBR322, by just making some slight changes in it. Origin of replication and ampicillin resistant gene remains the same in pUC8, as in pBR322. Only few nucleotide sequences of …
pUC 8 confers resistance to ampicillin and complements defects in b-galactosidase production in appropriate host strains (JM83). Foreign DNA inserted at restriction endonuclease sites within the multiple cloning site result in the loss of . b-galactosidase complementation.