At the PV92 locus of chromosome 16, Alu is a 300 bp dimorphic insert that can either be present or absent. It does not encode a protein product and has lost the ability to transpose. It is specific to humans, and differences in genotype and allele frequencies between human populations are important tools in understanding evolution.

PV92 is a human‐specific Alu insertion on chromosome 16. The PV92 genetic system has only two alleles indicating the presence (+) or absence (‐) of the Alu transposable element on each of the paired chromosomes. This results in three PV92 genotypes (++, +‐, or ‐‐).

2001年1月1日 · The PCR amplification of the PV92 Alu insertion locus was performed in 30 cycles of 95°C for 1 min, 54°C for 1 min, and 72°C for 1 min, with a final elongation step of 72°C for 7 min.

In the current model, the rt enzyme produces a nick at a chromosomal locus containing the sequence AATTTT. The polyadenylated "tail" of the Alu transcript (-AAAA) then hydrogen bonds to the TTTT sequence at the nick site, creating a primer for reverse transcription.

One example of a non-disruptive Alu element in humans is found in the location called PV92 on chromosome 16. This element is of the youngest subfamily of Alu, called Ya5. Since PV92 does not cause any deleterious effects, it can be used as a non-selected marker to illustrate lineage.

2009年9月11日 · PV92 is the only locus with a particularly high δ value in the comparison between the geographic groups ASI/EUR.

2017年3月4日 · A derivative of the cytoplasmic 7SL RNA, a major component of the signal recognition particle the Alu gene insertion is a retro-transposable genetic element without expression that resides as a...

To investigate H-W equilibrium, we will determine our genotypes for PV92 locus of chromosome 16. PV92 is a non-coding locus; as a result, no selective pressure should influence genotypic frequencies. Furthermore, because PV92 is a non-coding region, mating with respect to this locus should be random.

This experiment examines a human Alu dimorphism at the PV92 locus. A sample of human cells is obtained by saline mouthwash (alternatively DNA may be isolated from hair sheaths).

10: Polymerase Chain Reaction (PCR) locus PV92 Polymerase Chain Reaction or PCR allows important amplification (a million times) of a single DNA fragment. This method is widely used in molecular biology, medical diagnosis, the determination of microorganisms and even criminology. Kary Mullis, in the 1980s, invented the PCR.