Amplicon transgenes from potato virus X (PVX) are based on a modified version of the viral genome and are efficient activators of post-transcriptional gene silencing (PTGS). To determine whether...

2023年3月2日 · Among four lines transformed with Pikobody Enhancer, three specifically respond to GFP, accumulate Pikm-2 and Pikm-1 Enhancer proteins, and reduce virus load of PVX-GFP but

2021年11月17日 · PVX has been at the forefront of important advances in plant biology, such as the antiviral gene silencing machinery, the development of viral vectors to express foreign genes, the use of the green fluorescent protein (GFP) to track plant virus infection, discovering the role of viral movement proteins in plasmodesmata transport, and cloning of ...

2009年10月25日 · Protoplasts were prepared from BY-2 suspension cells and inoculated with PVX–GFP (Figs. 3 A–E) or PVX.TGBp3–GFP (Figs. 3 H–M). PVX–GFP shows cytosolic and nuclear fluorescence that does not change over time. PVX replicase was detected in vesicles surrounding the nucleus and throughout the periphery of the cell.

2024年9月18日 · We aimed at testing the impact of exogenous applied dsRNA on both layers of defence against potato virus X expressing GFP (PVX-GFP) in Nicotiana benthamiana. Co-inoculation of PVX-GFP with either sequence-specific (RNAi) or nonspecific dsRNA (PTI) showed that nonspecific dsRNA reduced virus accumulation in both inoculated and systemic leaves.

2005年7月29日 · The green fluorescent protein (GFP) gene was fused to the potato virus X (PVX) TGBp2 gene, inserted into either the PVX infectious clone or pRTL2 plasmids, and used to study protein subcellular targeting.

2021年2月15日 · Plants were syringe inoculated with agrobacteria carrying the constructs PVX-GFP and PVX-GFP NABP. GFP fluorescence is shown for several systemic leaves.

2022年5月20日 · We have developed a Potato virus X (PVX)-based vector system compatible with the GoldenBraid 2.0 (GB) cloning strategy to transiently express heterologous proteins or peptides in plants for biotechnological purposes.

本氏烟草通过从受基于 PVX 的载体感染的叶外植体的芽诱导获得具有重组 GFP 瞬时表达的体外植物组织培养物。 由于携带靶基因的病毒 RNA 的自然全身分布，瞬时表达发生在新组织和再生体中。 在体外生长的植物中基因沉默被延迟，并且在植物中检测到 GFP 持续了五到六个月。 不含农杆菌、表达 GFP 的植物可以在体外微繁殖（避免农杆菌渗透步骤），通过再生（保持培养多年）“恢复活力”，或转移到土壤中。 再生体中的平均 GFP 为总可溶性蛋白 (TSP) 的 18% (0.52 mg/g …

For VIGS of GFP by using PVX-GFP, silencing is maintained in the absence of vi- rus accumulation, whereas for PVX-35S and PVX-rbcS, si- lencing of the corresponding nuclear sequence always...