2020年3月9日 · In this study, we have covalently modified a specific nucleophilic cysteine residue of the PYP-tag protein with weakly fluorescent α,β-unsaturated ketone (conjugate addition) …

We developed novel fluorogenic probes for no-wash live-cell imaging of proteins fused to PYP-tag, which is a small protein tag recently reported by our group. Through the design of a new …

2019年7月29日 · One such chemical tag is the photoactive yellow protein (PYP)-tag, which is a small bacterial protein, developed for the selective labelling and imaging of proteins. Herein, …

In this study, we have developed a new protein-labeling probe that employs an ‘OFF–ON–OFF’ fluorescence switch to enable real-time imaging of the expression (fluorescence ON) and …

2018年1月30日 · To develop a synthetic-molecule/protein hybrid probe in living cells, we employed a PYP-tag-based system that we previously developed for specific labeling of …

2016年8月31日 · Here we reviewed the recent development of no-wash small molecular probes for photoactive yellow protein (PYP-tag), by the means of utilizing a quenching mechanism …

2015年12月28日 · This paper presents Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST), a small monomeric protein tag, half as large as the green fluorescent protein, …

2023年8月28日 · The PYP-tag, developed by the Kikuchi group in 2009, is a 14 kDa self-labeling tag employing the photoactive yellow protein (PYP) as the protein tag, which is naturally found …

2020年4月8日 · The best DMAC-CMK probe enabled rapid no wash labeling of the PYP-tag, with the remarkable stability of its PYP-tag conjugates due to the formation of a strong thioether …

2016年8月22日 · Here, to clarify the role of the GLUT4 glycosylation, we developed fluorogenic PYP-tag probes with multiple fluorescence colors and rapid kinetics.