(Empty Backbone) pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

In the first assembly step (pBIG1 vector level), up to five GECs are combined in a Gibson assembly reaction with one of five pBIG1 vectors, called pBIG1a–pBIG1e. Importantly, the “last” GEC—that is, the one that will occupy the most 3′ position in the pBIG1 construct—has to carry the ω linker sequence (introduced by oligonucleotide ...

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes. Proc Natl Acad Sci U S A. 2016 May 10;113 (19):E2564-9. doi: 10.1073/pnas.1604935113. Epub 2016 Apr 25. PubMed Article. How was your experience today? We store and distribute high-quality plasmids from your colleagues.

pBIG1a载体价格质粒购买价格是1200元，pBIG1a载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄霉素和大观霉素，质粒大小为5915 bp，测序引物为TCAACAGGTTGAACTGCTGATC/GGTGTAGCGTCGTAAGCTAATAC。

2018年4月1日 · Generated pLIB constructs can be used as templates for GEC amplification and multigene construct generation in pBIG1 or for transfer of its GEC to a baculoviral genome using Tn7 transposition (Tn7R, Tn7L sites and gentamicin resistance). pLIB …

提供 pBIG1a 载体/质粒图谱，全长序列，抗性，大小和元件信息。

2025年3月14日 · 上海海吉浩格生物科技有限公司 版权所有 技术支持：创世网络 . 地址： 合肥市恒山路桃花智谷嘉创业园5号202-1 电话：17031145927. q q/微信：1822235623. 邮箱：[email protected]

2016年5月10日 · Here we describe a method that allows up to 25 cDNAs to be assembled into a single baculoviral expression vector in only two steps. This method, called biGBac, uses computationally optimized DNA linker sequences that enable the efficient assembly of linear DNA fragments, using reactions developed by Gibson for the generation of synthetic genomes.

产品名称：pBIG1a,货号：HZB827202,用途:蛋白表达,宿主:昆虫细胞; 灰藻生物专注于微生物菌种和分生物资源开发,微生物资源鉴定保藏平台,咨询电话027-81388188

Transform pFastBac or pBIG1a vector containing expression cassettes into DH10EMBacY E. coli competent cells (Geneva Biotech). This enables transposase-mediated recombination resulting in a bacmid containing expression cassettes (Bac-to-Bac Baculovirus Expression System, Thermo Fisher Scientific).