Bacterial vector with a CloDF13 (CDF) origin for the co-expression of two genes. Sequence Author: MilliporeSigma (Novagen) Explore Over 2.7k Plasmids: pET & Duet Vectors …

pCDFDue-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR).

2020年6月13日 · Duet-1系列载体被设计用来在大肠杆菌中同时表达两个外源基因. 载体含有两个多克隆位点，每一个多克隆位点是通过T7启动子/Lac操纵子和一个核糖体结合位点组成。 大肠杆菌蛋白双表达载体，有两个多克隆位点 Duet-1系列载体被设计用来在大肠杆菌中同时表达两个外源基因. 载体含有两个多克隆位点，每一个多克隆位点是通过T7启动子/Lac操纵子和一个核糖体结合位点组成。 大肠杆菌…

pCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs).

2022年2月8日 · pCDFDuet-1质粒载体的图谱及使用说明，pCDFDuet™-1是为两个目标ORF的共表达而设计的。 该载体包含两个多克隆位点（MC），每个位点前面都有一个T7lac启动子和核糖体结合位点（rbs）。

pCDFDuet-1质粒是一种原核表达载体， N端含有一个6×His标签，C端含有一个S标签。 该质粒含有多个常用的酶切位点，便于不同基因克隆。 表达由宿主细胞提供的T7 RNA聚合酶诱导，目的基因被克隆到质粒载体上，受噬菌体强转录及翻译信号控制。 该质粒还携带有CloDF13复制因子、lacI基因和链霉素抗性基因。 可以使这个pETDuet-1载体在一个适当的宿主菌株的coexpression 4目标基因插入MCS1的基因可以使用ACYCDuetUP1引物和DuetDOWN1引物进行测序。 插 …

pCDFDuet™-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR).

Plasmid pCDFDuet-1:ChrH-ChrI from Dr. Wilfred van der Donk's lab contains the insert ChrH and is published in ACS Cent Sci. 2023 Apr 24;9 (5):1008-1018. doi: 10.1021/acscentsci.3c00160. eCollection 2023 May 24. This plasmid is available through Addgene.

提供 pCDFDuet-1 载体/质粒图谱，全长序列，抗性，大小和元件信息。

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker.