2016年11月1日 · Here, we report a novel biosensor based microscopy approach termed Photonic Crystal Enhanced Microscopy (PCEM) that enables the movement of cellular materials at the plasma membrane of individual live cells to be dynamically monitored and quantitatively imaged.

Here, we report a novel biosensor based microscopy approach termed Photonic Crystal Enhanced Microscopy (PCEM) that enables the movement of cellular materials at the plasma membrane of individual live cells to be dynamically monitored and quantitatively imaged.

To quantify live-cell adhesion, a photonic crystal biosensor surface with an extracellular matrix coating is monitored within a PCEM instrument to dynamically image changes in attached cell mass density during live stem cell attachment, spreading, and drug response.

We introduce photonic crystal enhanced microscopy (PCEM) as a label-free biosensor imaging technique capable of measuring cell surface attachment and attachment modulation. The approach uses a photonic crystal optical resonator surface incorporated into conventional microplate wells and a microscope-based de

PCem最初是英国开发者Sarah Walker于2007年8月15日发布的一款PC仿真免费开源软件，采用C及C++编写而成。 它最初是作为IBM PC XT模拟器开发的，以后在作者本人以及众多贡献者的共同努力下，逐步实现了模拟众多型号IBM 兼容PC的功能。

Photonic Crystal-Enhanced Microscopy (PCEM) is a powerful multimode imaging approach that combines label-free (LF) and enhanced-fluorescence (EF) imaging modalities for quantification and visualization of the interactions between cells and their substrate.

Here, we report a novel biosensor based microscopy approach termed Photonic Crystal Enhanced Microscopy (PCEM) that enables the movement of cellular materials at the plasma membrane of individual live cells to be dynamically monitored and quantitatively imaged.

In a recent publication [1], we demonstrated photonic crystal enhanced microscopy (PCEM) as a label-free biosensor-based cell attachment imaging approach that quantifies cell-surface interactions with spatial resolution sufficient for monitoring intra-cell attachment distribution, and temporal resolution sufficient for generating time-lapse ...

[4] 3DFX Voodoo Graphics：PCem用软件来模拟这个。 由于新的动态重编译器，仿真速度比v10快得多（感谢它），当使用两个渲染线程时，大多数计算机应该能达到Voodoo 1的表现。

The emulation is a lot faster than in v10 (thanks to a new dynamic recompiler) and should be capable of hitting Voodoo 1 performance on most machines when two render threads are …