2021年4月20日 · Plasmid pCK-D390TN(UniProt)-FLAG from Dr. David Bartel's lab contains the insert N-terminally truncated transdominant negative Drosha and is published in Mol Cell. …

2022年6月4日 · Two hundred micrograms of pCK or pCK-HGF-X7 was intramuscularly introduced simultaneously with CCI surgery. The affected L4-6 DRG neurons were isolated 4 days later, …

2019年9月24日 · Prepare aliquots for DNA parts to be assembled at a concentration of 15 nM and of the pCk vector at a concentration of 7.5 nM. To calculate the concentration needed for each …

I need to know the structure and full length sequence of this plasmid. Yes i did try addgene. but could not find anything. Lookup Snapgene map repository. Got a technical question? Get high …

Loop fusion sites in the pCk v ectors to assemble different L1 constructs int o a L2 construct using a pCs v ector and SapI are: a (ATC), b (GCA), d (TAC), e (CAG) and o (GCT). Left (LB) and …

Protocol based on: Pollak B, Cerda A, Delmans M, et al (2019) Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol 222:628–640 https...

2015年12月8日 · VM202 (pCK-HGF-X7) is a plasmid DNA that contains a genomic-cDNA hybrid human HGF sequence designed to simultaneously express two HGF isoforms. 6 Compared …

2000年5月27日 · We have constructed an expression vector, pCK, that is able to drive high levels of gene expression in the skeletal muscles of mice. pCK contains not only the full length …

2010年2月27日 · The paired product in pTS now has flanking activation tags and becomes an L or R sub-fragment for downstream assembly in a pCK vector. The digestion–ligation–selection …

2003年8月8日 · As a plasmid expression vector for intramuscular gene therapy, we used pCK, which has been shown to drive a high level of gene expression in the skeletal and cardiac …