本文综述了 DGAT 与 PDAT 基因的国内外研究进展，重点总结了二者在油料植物油脂合成中的生物学功能，在逆境胁迫下影响植物脂质代谢与生长发育的分子机制，以及合成生物学背景下 DGAT 和 PDAT 基因在驱动油脂合成中的重要作用，同时对深入开展 DGAT 和 PDAT 基因的机理研究与应用进行了展望，为深入了解植物油脂合成的分子机制，利用 DGAT 和 PDAT 基因改良油料作物品质、提高油料产能提供了依据。 There is a large gap between production and demand of …

2000年5月30日 · This reaction is catalyzed by an enzyme that we call phospholipid:diacylglycerol acyltransferase, or PDAT. PDAT was characterized in microsomal preparations from three different oil seeds: sunflower, castor bean, and Crepis palaestina. We found that the specificity of the enzyme for the acyl group in the phospholipid varies between these species.

2023年7月26日 · Here, we focus on hitherto unknow, new promising cold-stimulated function of phospholipid:diacylglycerol acyltransferase1 (PDAT1) – an enzyme catalyzing the last step of triacylglycerol (TAG) biosynthesis. Overexpression of AtPDAT1 boosted seed yield by 160% in Arabidopsis plants exposed to long-term cold compared to standard conditions.

2012年9月25日 · We present detailed genetic, biochemical, functional, and physiological analyses of phospholipid:diacylglycerol acyltransferase (PDAT) in the green microalga Chlamydomonas reinhardtii, which catalyzes TAG synthesis via two pathways: transacylation of diacylglycerol (DAG) with acyl groups from phospholipids and galactolipids and DAG: DAG transacy...

We present detailed genetic, biochemical, functional, and physiological analyses of phospholipid:diacylglycerol acyltransferase (PDAT) in the green microalga Chlamydomonas reinhardtii, which catalyzes TAG synthesis via two pathways: transacylation of diacylglycerol (DAG) with acyl groups from phospholipids and galactolipids and DAG:DAG ...

2016年5月24日 · In addition to the Kennedy pathway for de novo biosynthesis, triacylglycerol (TAG), the most important stock for microalgae-based biodiesel production, can be synthesized by phospholipid:...

2023年9月27日 · Phospholipid:diacylglycerol acyltransferase (PDAT) plays a key role in the acyl-CoA-independent pathway by transferring acyl-group from phospholipids (PL) to diacylglycerol (DAG) to from TAG. In thraustochytrid Aurantiochytrium sp. SD116, an active Au PDAT was confirmed by heterologous expression in a TAG-deficient yeast strain H1246.

2024年1月31日 · In the acyl-CoA-independent pathway of triacylglycerol (TAG) synthesis unique to plants, fungi, and algae, TAG formation is catalyzed by the enzyme phospholipid:diacylglycerol acyltransferase (PDAT). The unique PDAT gene of the model diatom Phaeodactylum tricornutum strain CCMP2561 boasts 47 single …

2013年10月7日 · Phospholipid:diacylglycerol acyltransferase (PDAT) and diacylglycerol:acyl CoA acyltransferase play overlapping roles in triacylglycerol (TAG) assembly in Arabidopsis, and are essential for seed and pollen development, but the functional importance of PDAT in vegetative tissues remains largely unknown.

2025年1月3日 · PDAT enzymes use membrane phospholipids as direct fatty acid donors to generate triglycerides stored in lipid droplets. This study identifies an inhibitory segment within the budding yeast PDAT Lro1, and demonstrates that constitutive activation of Lro1 antagonizes ER membrane expansion.