pdest15 Gateway® destination vector for inducible high-level expression of N-terminally GST-tagged proteins in bacterial cells. Sequence Author : Thermo Fisher (Invitrogen)

pDEST15载体价格1500元，pDEST15载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Ampicillin，质粒大小为7013bp，测序引物为T7-F/R，是Gateway系统的pDEST15载体。

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins.

Gateway destination vector for inducible high-level expression of N-terminally GST-tagged proteins in bacterial cells. BL21-AI expresses T7 polymerase induced by arabinose and recognizes the T7 promoter, so expression of the protein must be induced by adding arabinose to the medium. 1. 发货形式：质粒干粉 (常温运输，存于-20度，请务必先转化提质粒后使用) 2.

Plasmid pDEST15-GST-C11orf83 from Dr. Amos Bairoch's lab contains the insert UQCC3 WT and is published in Mol Cell Biol. 2015 Apr;35 (7):1139-56. doi: 10.1128/MCB.01047-14. Epub 2015 Jan 20. This plasmid is available through Addgene.

为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在大肠杆菌、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白 了解更多信息. Have Questions? 为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在 大肠杆菌 、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白。 无论是入门克隆还是 Ultimate™ RF 克隆，所有 Gateway™ 目的载体具有使用任何 att L-侧区片段 …

LOCUS pDEST15 Gateway 7013 bp DNA SYN DEFINITION pDEST15 Gateway ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.

pDEST15载体基本信息： BL21-AI在阿拉伯糖诱下表达T7聚合酶，识别T7启动子，因此表达蛋白时必须向培养基中加入阿拉伯糖进行诱导。 pDEST15载体质粒图谱和多克隆位点信息： pDEST15载体简介. pDEST15载体序列： ORIGIN. 1 ATCGAGATCT CGATCCCGCG AAATTAATAC GACTCACTAT AGGGAGACCA CAACGGTTTC. 61 CCTCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGTCC CCTATACTAG. 121 …

2011年8月23日 · The pDEST15 Gateway destination vectors were created for the rapid cloning with a Gateway entry clone using lambda phage site-specific recombination. All of the vectros contain T7 promoters and pBR322 origins of replication to help reduce the basal expression levels in …

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