Bacterial vector with an ampicillin resistance marker, for regulated expression of recombinant proteins with a cleavable 6xHis-SUMO tag.

Plasmid pCMV-PEmax from Dr. David Liu's lab contains the insert PEmax and is published in Cell. 2021 Oct 28;184 (22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018. This plasmid is available through Addgene.

pE-SUMO是原核表达载体。

The pE-SUMOstar vector is for the expression of recombinant SUMOstar fusion proteins (containing an N-terminal His6 tag) in E. coli. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with kanamycin resistance facilitating plasmid selection and stability.

Linearized bacterial vector with 3'-T overhangs for TA cloning and inducible expression of an N-terminally 6xHis- and SUMO-tagged protein. Sequence Author : Thermo Fisher (Invitrogen) Open in SnapGene

Streamlined bacterial cloning vector with an ampicillin resistance marker. Sequence Author: Eurofins Genomics. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different BanII sites may not be compatible. Prolonged incubation with NdeI may lead to removal of additional nucleotides.

2020年10月6日 · In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize.

The pE-SUMOpro vector is for the expression of recombinant SUMO fusion proteins (containing an N-terminal His6 tag) in E. coli. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with ampicillin resistance facilitating plasmid selection and stability.

2024年1月29日 · Prime editing minimally requires a prime editor (PE) protein—a fusion of a catalytically impaired nickase Cas9 (nCas9) and a reverse transcriptase (RT) enzyme—and a prime editing guide RNA...

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