Cloning and expression of pectinase gene from two forest soil …
2023年12月1日 · The pectinase gene from two soil isolates, identified as Streptomyces sp. (S-5 and S-14), was successfully cloned and expressed in a very common expression vector, E. coli BL21 (DE3). Both expressed pectinases, PelA-S5 and PelA-S14, showed maximum activity at 50 ℃ and in two pH (5 and 9).
A pectinase dose of 500 µl enzyme with 1 g of fruit pulp exhibited optimum release of reducing sugar and decrease in viscosity (54 %) at pH 7.0, after 24 h of treatment at 37ºC23. A total of 70 bacterial strains were isolated from 12 soil samples and screened for pectinase production by spot inoculation on Vincent’s agar medium containing ...
Demonstration of Pectinase Enzyme Activity from Soil Isolated …
2019年9月1日 · One unit of Pectinase activity was defined as the amount of enzyme which liberated 1μm glucose per min. Five bacterial isolates were obtained from soil containing decaying fruits and vegetables. The criterion for the selection process was based on isolation of species with similar morphological features for the test standard culture containing ...
Screening and Molecular Identification of Pectinase Producing …
2018年4月3日 · Emerging new applications underline the importance of screening pectinase producing microorganisms with novel properties, greater enzyme activity, and large-scale production of these enzymes . In this study, the isolates were subjected into plate agar and submerged fermentation screening methods to identify potent isolate with highest enzyme ...
es of cell wall [9]. Pectinase group of enzymes include polygalacturanases, pectin methyl esterase, pectin lyases. These pectinase enzymes act in different ways and on the pectins. Pectinase are extensive. soil and screen the pectinase producing bacteria as well as used cheaper substrate to enhance the pectinase enzyme prod.
Production and characterization of extracellular pectinase from …
2021年1月1日 · Pectinase was produced by submerged fermentation and the purified. The purified enzyme demonstrated 3.40 mg/ml of total protein and 484.70 U/mg of specific activity. In characterization studies, the pectinase demonstrated good activity at pH 6.0 and 40 °C.
Production and characterization of extracellular pectinase from a …
2021年1月1日 · The study aimed to identify pectinolytic bacteria in fruit waste soil and study the characteristics of enzyme and bacterial isolate for obtaining commercially importance pectinase with high activity. Section snippets
Isolation, Screening, and Identification of Pectin Degrading ... - JCHR
2024年3月12日 · Microorganisms and plants serve as the primary sources of pectinase enzymes, with microbial pectinases gaining preference in industries due to economic considerations. This study aimed to isolate, screen, and identify pectin-degrading bacteria from diverse soil samples.
Exopolygalacturonase Production from the Novel Strain …
4 天之前 · A pectinase-producing strain, Lichtheimia sp. X-8, was isolated from the soil for the first time. Subsequently, Lichtheimia sp. UV-16, with a 1.23-fold increase in pectinase activity, was obtained via UV mutagenesis, and optimization of its liquid fermentation process boosted pectinase activity from 455.6 ± 12.7 to 3202.0 ± 82.1 U/mL. The crude enzyme was purified by salting out and anion ...
Isolation and Screening of Pectinase Producing Bacteria from Soil ...
Pectinase activity among twelve different fungal strains, Aspergillus niger IM09 was identified as a potential one to produce maximal level 831 U/g at pH 4.0. Media composition, incubation temperature, incubation time, substrate concentration, aeration, inoculum size, assay temperature and nitrogen sources were found to effect pectinase activity.