2020年8月17日 · In porcine epidemic diarrhea virus (PEDV), the only gene at this location, ORF3, encodes a 224-residue membrane protein shown to exhibit ion channel activity and to enhance virus production. However, little is known about its intracellular trafficking or about its function during PEDV infection.

2012年2月17日 · ORF3 protein can be detected in PEDV-infected cells and it functions as an ion channel in both Xenopus laevis oocytes and yeast. Mutation analysis showed that Tyr170 in TM4 is important for potassium channel activity. Furthermore, viral production is reduced in infected Vero cells when ORF3 gene is silenced by siRNA.

为揭示pedv orf3细胞质定位信号，本研究构建了pedv dr13野毒株(dr13 wt) orf3蛋白全长或系列orf3蛋白截短肽的egfp融合表达载体，利用激光共聚焦显微镜分析重组orf3蛋白及其系列截短肽在细胞内的分布，研究结果将为进一步研究orf3蛋白的细胞内转运及功能提供参考。

2012年2月17日 · ORF3 protein can be detected in PEDV-infected cells and it functions as an ion channel in both Xenopus laevis oocytes and yeast. Mutation analysis showed that Tyr170 in TM4 is important for potassium channel activity. Furthermore, viral production is reduced in infected Vero cells when ORF3 gene is silenced by siRNA.

The single accessory gene of porcine epidemic diarrhea virus (PEDV), ORF3, is dispensable for virus replication in vitro, while viral mutants carrying ORF3 truncations exhibit an attenuated phenotype of which the underlying mechanism is unknown.

【方法】 利用基于RNA同源重组的PEDV反向遗传学操作技术拯救一系列携带不同 orf3 基因及 orf3 基因缺失的重组PEDV；将获得的重组PEDV以MOI 0.1感染Vero细胞，分别于感染的第8、16、24、32、40、48 h测定其TCID 50 并绘制病毒生长曲线；分别在感染25 h和36 h利用全自动细胞计数分析仪对6孔板内的细胞进行计数，并于感染后的第12、24、36、48 h用CCK-8试剂盒对其细胞活力进行测定。 【结果】 RT-PCR结果及细胞病变观察证明成功拯救到了携带不同 orf3 基因 …

2023年6月1日 · PEDV ORF3 inhibits both poly (I:C)- and IFNα2b-stimulated transcription of IFN-β and ISGs mRNAs. PEDV ORF3 inhibits the type I signaling via down-regulating protein expression in the RLRs-mediated pathway. Our study for the first time describes a new mechanism by which PEDV ORF3 inhibits host innate antiviral responses.

PEDV ORF3 is a transmembrane protein that localizes at endoplasmic reticulum to induce ER stress response. PEDV ORF3 protein has no effect on apoptosis, but induces autophagy dependent on ER stress response. This work provides some new findings for the biological function of the PEDV ORF3 protein.

2015年11月4日 · Our results demonstrated that the ORF3 gene of PEDV could prolong S-phase, facilitate formation of vesicles and thus promote the proliferation of PEDV. These findings have potentially important implications for understanding the molecular mechanisms of …

2023年2月28日 · In this review, we summarize current knowledge and identify gaps in many aspects of ORF3 protein in PEDV, with emphasis on its unique biological features, including membrane topology, Golgi retention mechanism, potential intrinsic disordered property, functional motifs, protein glycosylation, and codon usage phenotypes related to genetic evoluti...