To develop and evaluate a two-dimensional LC system with charged aerosol detection for the simultaneous characterization of protein PEGylation and unreacted PEGylation reagent. The use of polyethylene glycol (PEG) as a safe and low cost additive for the pharmaceutical and cosmetic industries is common practice.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, PEG 200, PEG 400. Polyethylene glycol (PEG) has a low toxicity and is used in a variety of products (creams, laxatives, lubricants). When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood.

分析样品在0.2g-4g（精确至0.0002）和一定量的聚乙二醇（PEG）标准样品上称重。 用流动相溶解后，将样品定量移入25ml容量瓶中，用流动相稀释至刻度摇匀滤液用0.45μm滤膜过滤，滤液用于高效液相色谱仪的分析。

本应用简报描述了一种联用Agilent HPLC 及Agilent 6520 精确质量数Q-TOF LC/MS 系统分析聚乙二醇及聚乙二醇化蛋白的方法。 其使用了一种 电荷剥离剂（三乙胺）作为柱后添加剂，这样有助于简化质谱图，因此可更好地阐释结 果。

1998年3月27日 · Derivatisation efficiency for PEGs by dinitrobenzoyl chloride and quantitative determination of derivatised PEGs by HPLC have been studied. A reversed-phase (RP) mode of HPLC was examined for determination of PEG 400 oligomers. The normal-phase system provided greater resolution for oligomers of PEGs.

HPLC is a powerful analytical technique that is widely used for the measurement of PEG, PEGylated small drugs, and PEGylated liposomes and micelles (Table 3). HPLC analysis of clinical samples usually requires pretreatment steps to reduce sample complexity.

2020年10月1日 · Polyethylene glycol (PEG) are widely applied in detergents, cosmetics, and food additives. A simultaneous analytical method was developed to detection the polyethylene glycol (100–10000 Da). High-performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) could analyze successively PEG products.

2019年8月9日 · Description This application shows a method for HPLC-CAD analysis of polyethylene glycol (PEG). PEG is a polyether compound – a polymer of ethylene glycol. A number of PEGs exist based on molecular weight and geometry. PEG has many uses. In the food industry, PEG is used as an antifoaming agent, carrier, emulsifier, glazing agent and thickener.

本文建立了一种分析吐温 20 的RP-HPLC-ELSD 方法,该方法在直接进样 PEG 偶联的蛋白注射剂后,即可对其中的辅料吐温 20 进行准确的定量分析。 吐温 20 购自南京威尔药业股份有限公司(注射级);聚乙二醇(PEG,MW20000) 购自 JenKem Technology。 三氟乙酸 (TFA) 为 HPLC 级,购自 DikmaPure 公司;乙腈为 HPLC 级,购自 J.T. Baker 公司(美国)。 高纯水来自 Milli-Q 纯水系统(美国)现 制备。 含吐温 20 的PEG 偶联重组蛋白注射剂、不含吐温 20 的阴性样品和不含吐温 20 …

2017年10月17日 · This chapter describes a method for the quantitation of polyethylene glycol (PEG) in PEGylated colloidal gold nanoparticles using a reversed-phase high-performance liquid chromatography (RP-HPLC) with charged aerosol detection. The …