Polyethylene glycol (PEG)-mediated cell fusion is a simple and efficient technique used widely for the production of somatic cell hybrids and for nuclear transfer in mammalian cloning. We describe a basic protocol of PEG-mediated cell fusion for the production of somatic cell hybrids.

2017年12月1日 · Our new understanding on the biological interactions between PEG and cells sparks new molecular insights on the mechanism which underlies the good biocompatibility of PEG. Importantly, polymers can affect cell growth in various ways. For example, Poly(l-lactic acid) (PLLA) can cause enlarged mitochondria and glycogen accumulation [63].

1999年8月1日 · Poly (ethylene glycol) (PEG) is widely used in biology and biochemistry to precipitate molecules, to stabilize or to aggregate particles, and as vehicles for drug delivery. Its low toxicity, water solubility and ease to handle, render it the polymer of choice for many tasks. Yet, what makes PEG so multi-functional is not clear.

2009年2月3日 · PEG, a linear polymer of ethylene oxide with hydroxyl terminal groups [H– (O–CH2–CH2) n –OH], is a neutral, water soluble compound. In transplantation, PEG has been suggested to act as an immuno-camouflaging molecule that …

2023年4月15日 · Here, we investigated the effect of PEG on rat embryonic fibroblast 52 wild-type (REF52wt) cells, with a special focus on the influence that PEG has on cell stiffness, dry and total mass and cell morphology.

The mechanism by which polyethylene glycol (PEG) mediates cell fusion has been studied by examining the movements of membrane lipids and proteins, as well as cytoplasmic markers, from erythrocytes to monolayers of cultured cells to which they have been fused.

PEG ensures hydrophilicity, modulates interaction with plasma and cellular proteins, and lowers the antigenicity and immunogenicity of non-human therapeutic proteins. 1, 2 The application of PEG for drug modification has revolutionized the treatment of numerous diseases, including various therapeutics such as anticancer agents. 3 Furthermore ...

These studies elucidate PEG-mediated cellular signaling with the implication that the adhesion and apoptotic activity of PEG-rich materials can be sensitively controlled by anti-oxidant addition. Moreover, this study shows that biomaterials can drive the cell fate in opposing directions through concurrent property changes.

2025年3月5日 · Lungs of Ai14 tdTomato mice 4 days after transfection with PBAE-PEG/LNP and PBAE/LNP carrying 5moU Cre mRNA were harvested and sectioned for immunofluorescence staining to identify which lung cell types were transfected with Cre mRNA (12.5 μg administered twice on days 0 and 2), resulting in tdTomato expression as described in Figure 5. Cell ...

When MIN6 cells, a pancreatic β-cell line, were encapsulated in poly (ethylene glycol) (PEG) hydrogels, their survival and glucose responsiveness to insulin were highly dependent on the cell-packing density.