Vector for fusing EGFP to the N-terminus of a partner protein. For other reading frames, use pEGFP-C1 or pEGFP-C2.

pEGFP-C3 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-C3 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

pEGFP-C3编码野生型GFP（1-3）的红移变体，该变体已经过优化，可以在哺乳动物细胞中产生更亮的荧光和更高的表达。 （最大激发波长= 488 nm；最大发射波长= 507 nm。

pEGFP-C3 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-C3 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

Plasmid pEGFP-C3-hYAP1 from Dr. Marius Sudol's lab contains the insert YAP and is published in Mol Cell. 2003 Jan . 11 (1):11-23. This plasmid is available through Addgene.

pEGFP-C3-IRF3载体价格1500元，pEGFP-C3-IRF3载体质粒图谱 (Vector map)，载体序列 (Sequence)见下文，载体质粒抗性为卡那霉素，质粒大小为4727bp，测序引物为M13-F/R。

提供 pEGFP (C3)-Nup153 载体/质粒图谱，全长序列，抗性，大小和元件信息。

pEGFP-C3 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-C3 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

提供 pEGFP-C3 载体/质粒图谱，全长序列，抗性，大小和元件信息。

融合到EGFP的C端赋予融合蛋白的荧光特性，允许融合蛋白在体内定位。 靶基因应该被克隆到pEGFP-C3中，以使其与EGFP编码序列符合读框，而没有插入框内的终止密码子。 可以使用任何标准转染方法将重组EGFP载体转染到哺乳动物细胞中。 如果需要，可以使用G418筛选出稳定转化子。 pEGFP-C3也可以简单地用于在目标细胞系中表达EGFP（例如，作为转染标记）。 保存：-20℃保存，至少1年有效。 运输：冰袋运输。 1） 我司提供的载体/质粒/菌株都经过严格的质 …