Vector for fusing EGFP to the C-terminus of a partner protein. For other reading frames, use pEGFP-N2 or pEGFP-N3.

pEGFP-N1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

pEGFP-N1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

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pEGFP-N1编码野生型GFP（1-3）的红移变体，该变体已经过优化，可以在哺乳动物细胞中获得更亮的荧光和更高的表达。 （最大激发波长＝ 488nm；最大发射波长＝ 507nm。 ）pEGFP-N1编码GFPmut1变体（4），其包含Phe-64对Leu和Ser-65对Thr的双氨基酸取代。 EGFP基因的编码序列包含超过190个沉默碱基的变化，这些变化对应于人类密码子使用偏好（5）。 EGFP侧翼的序列已转化为Kozak共有翻译起始位点（6），以进一步提高真核细胞的翻译效率。 pEGFP-N1中 …

The pEGFP-N1 is a vector for fusing EGFP to the C-terminus of a partner protein. It has multiple cloning sites before the GFP gene.

pEGFP-N1-FLAG质粒价格1200元，pEGFP-N1-FLAG (addgene 60360 )质粒图谱（map），质粒序列 (Sequence)见下文，质粒抗性为卡那霉素，质粒大小为4733 bp，测序引物为CMV Forward/EGFP-N。 pEGFP-N1-FLAG质粒是哺乳细胞表达质粒。

pEGFP是一种优化的突变型GFP，使其产生的荧光较普通GFP强35倍，大大增强了其 报告基因 的 敏感度。 pEGFP与其他蛋白的 融合表达 已有很多成功的例子，而且其N及 C端 均可融合，并不影响其发光。 p EGFP -N1载体上携带有EGFP蛋白表达基因，如上图 质粒图谱 所示。 ④该载体具有neo基因，可以采用 G418 来筛选已成功转染了该载体的 靶细胞。 这些特殊的结构可以实现目的基因在靶细胞内的稳定表达。 pEGFP-N1载体是一种化学物质，为真核细胞表达载体，具有这 …

pEGFP载体价格3800元，pEGFP载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄青霉素，质粒大小为3355 bp，测序引物为EGFP-N。pEGFP载体是细菌表达载体。

pEGFP-N1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.