Vector for fusing EGFP to the C-terminus of a partner protein. For other reading frames, use pEGFP-N1 or pEGFP-N3.

pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized forbrighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

What is virus associated DNA, and why do I have to order it? Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources. This vector is NOT available from Addgene and the database is no longer actively maintained. This vector is not available from Addgene.

pEGFP-N2是一种优化的突变型GFP，使其产生的荧光较普通GFP强35倍，大大增强了其报告基因的敏感度。 EGFP与其他蛋白的融合表达已有很多成功的例子，而且其N及C端均可融合，并不影响其发光。

提供 pEGFP-N2 载体/质粒图谱，全长序列，抗性，大小和元件信息。

Plasmid Emerin pEGFP-N2 (588) from Dr. Eric Schirmer's lab contains the insert Emerin and is published in J Cell Biol. 2011 Apr 4;193 (1):109-23. doi: 10.1083/jcb.201009068. Epub 2011 Mar 28. This plasmid is available through Addgene.

pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

提供 pEGFP-N2-NRSE4x-ohne-CMV 载体/质粒图谱，全长序列，抗性，大小和元件信息。

2022年2月16日 · pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.