ELISA-Peptide Assay Protocol - Cell Signaling Technology
ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.
A Novel Peptide ELISA for Universal Detection of Antibodies to …
2011年6月10日 · Here, we describe a peptide ELISA to detect antibodies to H5N1 virus by using synthetic peptide that comprises the amino acid sequence of this highly conserved and antigenic epitope as the capture antigen.
Peptide ligand-based ELISA reagents for antibody detection
2018年10月15日 · Mixing functionalized peptide ligand with nonspecific detection reagent produces specific ELISA antibody detection reagent. Nonspecific detection regent can be turned into many ELISA reagents using peptides specific for desired target antibodies.
Synthetic Peptide-Based ELISA and ELISpot Assay for Identifying ...
Enzyme-linked immunosorbent assay (ELISA) is an invaluable diagnostic tool to detect serum autoantibody binding to target antigen. To map the autoantigenic epitope (s), overlapping synthetic peptides covering the total sequence of a protein antigen are used.
A short history, principles, and types of ELISA, and our laboratory ...
These peptide/protein concentrations in biological fluids are measured using various methods, the most common of which is enzymatic immunoassay EIA/ELISA and which guide the clinicians in diagnosing and monitoring diseases that inflict biological systems.
Optimization of Peptide-Based ELISA for Serological Diagnostics…
Peptide-based ELISA using selected 30mer peptides consistently resulted in increased sensitivity relative to 20mer peptides (Figs. 2b and 3b). We next addressed whether mixing combinations of 30mer peptides would further improve sensitivity and specificity.
Full article: A Novel Click Chemistry-based Peptide ELISA Protocol ...
2022年3月2日 · Indirect ELISA using immunoreactive peptides conjugated to bovine serum albumin offers a reliable method that is complementary to standard plastics and plate readers. Peptides were coupled to a common carrier protein and tested in standard plastics to establish an optimized peptide ELISA protocol.
Peptide ligand-based ELISA reagents for antibody detection
2018年10月15日 · In an effort to enhance practical utility of such peptide ligands, we describe here a simple approach to turn such target antibody-specific peptide ligands into specific ELISA detection reagents.
Peptide ligand-based ELISA reagents for antibody detection
2018年10月15日 · Nonspecific detection regent can be turned into many ELISA reagents using peptides specific for desired target antibodies. ELISA assays utilizing these detection reagents exhibit excellent analytical properties supporting their practical utility.
Competition between bound and free peptides in an ELISA …
2006年5月31日 · We describe an ELISA-based method that can be used to identify and quantitate proteins in biological samples. In this method, peptides in solution, derived from proteolytic digests of the sample, compete with substrate-attached synthetic peptides for antibodies, also in solution, generated against the chosen peptides.
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