Bacterial vector for expression of N-terminally 6xHis-tagged proteins. Sequence Author: MilliporeSigma (Novagen) Explore Over 2.7k Plasmids: pET & Duet Vectors (Novagen) | More Plasmid Sets. ApoI is typically used at 50°C, but is 50% active at 37°C. The 1-base overhangs produced by AhdI may be hard to ligate.

pet14b,pet 14b载体价格800元，载体质粒图谱(Vector map),载体序列(Sequence)和多克隆位点见网页内容，抗性为Amp，大小为4671bp，测序引物为T7和T7t，含有N-His标签和N-thrombin蛋白酶切位点，Novagen公司出品，主要用来大肠杆菌蛋白表达.

What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, and why do I have to order it? Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

Plasmid pET14b – E. coli Exonuclease I from Dr. Charles Bell's lab contains the insert Exonuclease I and is published in Nucleic Acids Res. 2013 Jun;41 (11):5887-97. doi: 10.1093/nar/gkt278. Epub 2013 Apr 22. This plasmid is available through Addgene.

提供 pET-14b 载体/质粒图谱，全长序列，抗性，大小和元件信息。

The pET-14b vector (Cat. No. 69660-3) carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map.

The pET-14b vector carries an N-terminal His?Tag? sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map.

载体别名：pET14b, pet 14b. 质粒类型：大肠杆菌表达载体. 表达水平：高. 克隆方法：多克隆位点，限制性内切酶. 载体大小：4671bp. 5' 测序引物：T7. 3' 测序引物：T7-ter. 载体标签：N-His, N-thrombin. 载体抗性：Ampicillin (氨苄青霉素)

pET14b载体质粒图谱和多克隆位点信息. pET14b载体简介. The pET-14b vector (Cat. No. 69660-3) carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map.

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