pET-32a(+) Sequence and Map - SnapGene
Bacterial vector for expressing thioredoxin fusion proteins with an enterokinase site. For other reading frames, use pET-32b(+) or pET-32c(+).
Construction of pET-32 α (+) Vector for Protein Expression and ...
A pET-32 α (+) vector series is designed for expression of peptide sequences fused with the 109 aa Trx•Tag™ thioredoxin protein. It belongs to a kind of expression systems which can produce the desired protein when activated after it is inserted into the prokaryotic host cells.
pET-32a(+) pet32a质粒图谱、载体图谱、序列、价格、抗性、测 …
pET32系列载体是设计用来克隆构建和高水平融合表达表达带有109个氨基酸Trx标签的蛋白质序列。 将基因插入到载体的多克隆位点，能够获得融合表达蛋白质。 同时融合蛋白包含有His标签和S标签，这些可以用于蛋白表达检测和纯化过程。 载体的多克隆位点见上面的图谱。 需要注意的是图谱中的核苷酸编码顺序是以pBR322载体的复制方向为正方向，所以T7表达区域在图谱中是 …
pET-32a(+)-NRG1B1 - Addgene
Plasmid pET-32a (+)-NRG1B1 from Dr. Paul Burridge's lab contains the insert NRG1-beta 1 and is published in Stem Cell Reports. 2020 Jan 9. pii: S2213-6711 (19)30446-1. doi: 10.1016/j.stemcr.2019.12.007. This plasmid is available through Addgene.
Construction of pET-32 α (+) Vector for Protein Expression and ...
The construction of expression vector is a basic tool for biotechnology and production of desired proteins, this article summarized the construction of pET-32 α (+) vector techniques which are generally used in research laboratories.
Addgene: Vector Database - pET-32 a,b,c(+)
What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, and why do I have to order it? Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
pET-32a(+) DNA, Novagen, with 109aa Trx-tag Sigma-Aldrich
The pET-32a(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag thioredoxin protein (1). Cloning sites are available for producing fusion proteins also containing cleavable HIS TAG ® …
pET-32a(+) vector map and sequence - novoprolabs.com
Provide pET-32a(+) vector/plasmid map, full length sequence, antibiotic resistance, size and other information
Vector: pET-32a(+) | Genophore
The pET-32a(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein . Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® …
备注:pET32a载体用于表达可溶性，具有活性功能的蛋白;pET-32a(+)载体具有N端Thrombin （凝血酶）酶切位点，N端肠激酶酶切位点。 pET32啊，pET32b，pET32c载体的差异主要