Bacterial vector for expression of N-terminally S-tagged proteins with a thrombin site. For other reading frames, use pET-29b (+) or pET-29c (+). Sequence Author: MilliporeSigma (Novagen) …

pET-29a-c载体带有N-S标签和C-His标签。 本载体与pet25b载体相比，只有抗性标记不同。 独特的酶切位点在载体图谱中被标注出来。 需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。 ORGANISM other sequences; artificial sequences; vectors. http://www.biofeng.com/ source 1..5371. /organism="pET-29 a (+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator"

pET29a载体质粒图谱和多克隆位点信息. pET29a载体简介. The pET-29a-c (+) vectors carry an N-terminal S•Tag™/thrombin configuration plus an optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map.

我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。 所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。 确保质粒的关键元件正确，但是我们并不能保证实验结果。 页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。 (如果测过序，本页面一般会提供下载) 开放数据库中的大多数载体序列都没有被 …

The pET-29a-c (+) vectors carry an N-terminal S?Tag?/thrombin configuration plus an optional C-terminal His?Tag? sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 …

The maps for pET-29b(+) and pET-29c(+) are the same as pET-29a(+) (shown) with the following exceptions: pET-29b(+) is a 5370bp plasmid; subtract 1bp from each site beyond BamH I at …

Plasmid sortase A pentamutant (eSrtA) in pET29 from Dr. David Liu's lab contains the insert sortase A pentamutant and is published in Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404. doi: 10.1073/pnas.1101046108. Epub 2011 Jun …

Plasmid pET29-neoLux1.2 from Dr. Andy Yeh's lab contains the insert neoLux1.2 and is published in Chem. 2024 Nov 12. doi: 10.1016/j.chempr.2024.10.013 This plasmid is available through Addgene.

The pET-29a-c (+) vectors carry an N-terminal S•Tag™/thrombin configuration plus an optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 …

Here we present a modification of the widely used pET29 expression vector for use in rapid and straightforward parallel cloning via a gene replacement and Golden Gate strategy. The modification can be applied to other expression vectors for Gram-negative bacteria.