pET-46 Ek/LIC载体是用来快速定向PCR高通量质粒构建的载体。 本载体带有N-Nus标签和His标签，S标签。 独特的酶切位点在载体图谱中被标注出来。 需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。 T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。 质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表 …

Bacterial vector for ligation-independent cloning (LIC) to express 6xHis-tagged proteins with an enterokinase site. Sequence Author: MilliporeSigma (Novagen) Explore Over 2.7k Plasmids: …

The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-46 Ek/LIC Cloning Kit (Cat. No. 71335-3), inserts can be efficiently cloned without the need for restriction digestion or ligation.

The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-46 Ek/LIC Cloning Kit (Cat. No. 71335-3), inserts can be efficiently cloned without the need for restriction digestion or ligation.

The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source of T7 RNA polymerase in the host cell.

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The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-46 Ek/LIC Cloning Kit (Cat. No. 71335-3), inserts can be efficiently cloned without the need for restriction digestion or ligation.

The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-46 Ek/LIC Cloning Kit (Cat. No. 71335-3), inserts can be efficiently cloned without the need for restriction digestion or ligation.

2023年9月11日 · Here we report on the structural and biochemical characterization of PET46 (RLI42440.1), an archaeal promiscuous feruloyl esterase exhibiting degradation activity on semi-crystalline PET powder...

The pET-46 Ek/LIC Vector is a LIC-compatible derivative of pET-45b (+). The plasmid contains a strong T7 lac promoter and an amino-terminal His•Tag ® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence.