2025年3月31日 · The Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit provides a simple and direct procedure for precise measurement of PFK enzyme activity in various biological samples. This kit is based on a coupled enzyme assay, in which fructose-6-phosphate in the presence of ATP is converted to fructose1,6 …

磷酸果糖激酶（英語Phosphofructokinase；PFK）是一類激酶，可作用於果糖-6-磷酸。可分為兩種，分別產生不同產物： 由磷酸果糖激酶1作用可得果糖-1,6-雙磷酸。 由磷酸果糖激酶2作用可得果糖-2,6-雙磷酸。

2023年11月19日 · 11月14日，复旦大学上海肿瘤医院和复旦大学生物医学研究院周祥课题组在期刊《Cell Reports》上发表题为“p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP”的研究论文， 研究表明，CMBL通过抑制糖酵解来抑制CRC的生长，并提出了治疗CMBL缺陷CRC的潜在联合策略。 https://www.cell.com/cell-reports/fulltext/S2211-1247 (23)01438-9#%20. 研究背景. 01.

In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.

The Phosphofructokinase Colorimetric Assay kit provides a simple and direct procedure for measuring PFK activity in a variety of samples. PFK activity is determined by a coupled enzyme assay, in which fructose-6-phosphate and ATP is converted to fructose-1,6-diphosphate and ADP by PFK. The ADP is converted by the enzyme mix to AMP and NADH.

Phosphofructokinase (PFK) Activity Colorimetric Assay Kit (< 1 mU/well) 產品描述： 磷酸果糖激酶（Phosphofructokinase ,PFK）是一種關鍵的糖酵解酶，在糖酵解(glycolysis)過程中起著重要的調節作用。

ab155898 PFK Activity Assay Kit (Colorimetric) 1 1. Overview 6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898) provides a simple and sensitive method to measure total 6-phosphofructokinase (PFK) activity in cell and tissue extracts. The assay is based in the conversion by PFK of fructose-6-phosphate to

PFK activity is determined by a coupled enzyme assay, in which fructose-6-phosphate and ATP is converted to fructose-1,6-diphosphate and ADP by PFK. The ADP is converted by the enzyme mix to AMP and NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the PFK activity present.

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Phosphofructokinase (PFK) Activity Colorimetric Assay Kit. sufficient for 100 colorimetric tests. All Photos (3) NACRES: NA.84. Recommended Products. Slide 1 of 10. 1 of 10. Sigma-Aldrich. MAK408. Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric) sufficient for 100 colorimetric tests.