Pfu DNA Polymerase是一种来源于嗜热菌 Pyrococcus furiosus 的高度热稳定的DNA聚合酶。 Pfu酶的分子量为90kDa。 Pfu酶可以催化5'至3'方向的依赖于DNA模板的脱氧核苷酸的聚合。 和Taq酶不同，Pfu酶有3'至5'的外切酶活性 (proofreading activity)。 另外，Pfu酶有5'至3'外切酶活性，但Pfu酶没有反转录酶活性。 由于Pfu酶有3'至5'的外切酶活性，因此在PCR扩增过程中出错的几率大大降低，出错率为2.6×10 -6 per nt per cycle。

Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to copy the organism's DNA during cell division (thermostable DNA polymerase). In the laboratory setting, Pfu is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new ...

A protocol for use of an enzyme that replicates DNA at 75°C and is recommended for use in PCR and primer extension reactions that require high fidelity. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium.

Pfu DNA Polymerase is provided with an optimized 10X Pfu Buffer which includes MgSO 4 at a concentration of 20 mM. A final MgSO 4 This product is developed, designed and sold exclusively concentration of 2 mM is generally ideal for PCR. The MgSO 4 concentration can be optimised by using 10X Pfu Buffer and MgSO 4 solution provided in a separate ...

Pfu DNA polymerase, a proofreading DNA polymerase isolated from Pyrococcus furiosus, is an ideal choice for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).1–3 These applications include cloning, gene …

该产品是从高温嗜热菌Pyrococcus furiosis分离出的高保真耐高温DNA polymerase，分子量为90 kDa，具有3’→5’外切核酸酶活性，无5’→3’外切核酸酶活性，其扩增产物为平末端，无突出的A。 DNA聚合时延伸速度为600 bp/min；PCR产物为平末端。 其他高温DNA polymerase，如：Vent、DeepVent、Tli、UlTma等具有校正功能，但是Pfu是目前已发现的所有DNA polymerase中扩增DNA时出错率很低的。 常规 PCR、高保真 PCR、基因定点突变、 克隆表达。 每个包装包 …

体外实验中，在聚合酶链反应（polymerase chain reaction, PCR）中，使用Pfu聚合酶可以快速，高保真的扩增DNA片段。 Sso7d是来源于Sulfolobus solfataricus的一种双链DNA结合蛋白,与DNA聚合酶融合后,聚合酶的进行性得到了显著的提升,而其对聚合酶催化活性和热稳定性没有影响。

PCR过程有效升降温，PCR体积应尽可能缩小，但同 时需要考虑高温过程水分蒸发的问题，常见的PCR仪 和0.2 ml PCR管最佳体积为25-50 µl。 1. PCR成分准备 将10×pfu Buffer，dNTPs, 模板DNA和引物室温 解冻后置于冰上。10×pfu Buffer解冻后需充分混合均 匀。 2.

Pfu DNA Polymerase is a high-fidelity thermostable DNA polymerase that replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´-->3´ direction in the presence of magnesium. Also has 3'-->5' exonuclease (proofreading) activity for nucleotide incorporation with a …

Pfu DNA Polymerase 是从克隆有 Pyrococcus furiosis DNA Polymerase 基因的大肠杆菌中分离纯化的， Pfu DNA Polymerase 具有 5′→3′DNA 聚合酶活性和 3′→5′ 外切酶活性，能纠正 DNA 扩增过程中产生的碱基错配, 其扩增的 PCR 产物为平端。