If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.</strong><br /><br /><strong></strong>PhAM^floxed (photo-activatable mitochondria) mice conditionally express a mitochondrial-specific version of Dendra2 green/red photoswitchable ...

To investigate mitochondrial dynamics in tissues and disease models, we generated two mouse lines with photo-activatable mitochondria (PhAM). In the PhAM (floxed) line, a mitochondrially localized version of the photo-convertible fluorescent protein Dendra2 (mito-Dendra2) is targeted to the ubiquitously expressed Rosa26 locus, along with an ...

Exposure of mitochondria to 405 nm laser light irreversibly switches green fluorescence to red, enabling measurements of mitochondrial fusion and transport.

To investigate mitochondrial dynamics in tissues and disease models, we generated two mouse lines with photo-activatable mitochondria (PhAM). In the PhAMfloxed line, a mitochondrially localized version of the photo-convertible fluorescent protein Dendra2 (mito-Dendra2) is targeted to the ubiquitously expressed Rosa26 locus, along with an ...

为了研究组织和疾病模型中的线粒体动力学，我们生成了两个具有光激活线粒体 ( PhAM ) 的小鼠系。 在PhAM floxed线中，光可转换荧光蛋白 Dendra2 (mito-Dendra2) 的线粒体定位版本与上游loxP侧翼终止信号一起靶向普遍表达的Rosa26基因座。

In mice, removal of the neomycin selection marker by Flp recombinase results in the PhAMfloxed line (schematic 4), which can be mated to a Cre driver line to obtain cell-specific labeling of...

2024年1月17日 · Next, we identified that osteocytes communicated with cancer cells through mitochondrial transfer using an osteocyte-specific mitochondria reporter mouse model (DMP1-Cre PhAM). This intercellular mitochondrial transfer was mediated by Miro1 and MFN2 and declined with aging (along with the levels of MFN2).

2023年8月18日 · Hearts from aged mice or Danon disease patients have increased levels of secreted EVs containing mitochondria indicating activation of vesicular release during cardiac pathophysiology. Overall,...

2020年2月10日 · In a mouse model overexpressing dynamin-related protein 1 (Drp1) specifically in skeletal muscle (Drp/MyoD iCre (Drp/MC) mice), we found a reduction of muscle mass mainly caused by significant...

2012年7月23日 · In the PhAM floxed line, a mitochondrially localized version of the photo-convertible fluorescent protein Dendra2 (mito-Dendra2) is targeted to the ubiquitously expressed Rosa26 locus, along with an upstream loxP -flanked termination signal.