Auramine phenol staining of smears for screening acid fast ... - PubMed
Auramine stain showed high sensitivity (47.14%) and specificity (96.58%). Result of the present study showed that the auramine stain is a better method for screening samples from the suspected cases of tuberculosis sample especially pulmonary and extrapulmonary cases where bacilli count is usually low.
Evaluation of Ziehl-Neelsen staining, auramine phenol staining, …
The study was designed to determine the efficacy of modified Ziehl-Neelsen (ZN), Auramine-Phenol (AP) staining, antigen detection enzyme linked immunosorbent assay (ELISA) and nested polymerase chain reaction (PCR) for detection of cryptosporidia in 671 HIV-seropositive patients, 353 HIV-seronegative patients including 198 children with ...
Elimination of Phenol from the Auramine Fluorescence Staining Method ...
2013年7月18日 · This report describes the use of Liquid Organic Cleaner (LOC), a liquid detergent, to replace the toxic pheno1 in the auramine O fluorescence method for the demon-stration of acid-fast bacilli. The modified method yields similar results, but offers several advantages over the method involving phenol as lipophilic agent.
Optimisation of acid fast smears for the direct detection of
The auramine phenol stain applied to a liquefied, concentrated sample and examined before the decontamination process is the most effective method for the demonstration of acid fast organisms in sputum.
Auramine-Phenol vs. Modified Kinyoun’s Acid-Fast Stains for …
2014年2月1日 · Auramine-phenol stain was compared with Kinyoun’s acid-fast stain to detect coccidia parasites in fecal samples from immunocompromised patients. The comparison was based on the number of detected cases, sensitivity, specificity, time required for the procedure, ease of use, interpretation, and cost.
Auramine-Phenol Stain: Introduction, Principle
2023年2月20日 · Auramine-Phenol is a fluorochrome stain used to visualize acid-fast structures of various microorganisms, especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , Isospora belli, and fungal spores.
Auramine Staining: Introduction, Principle, Procedure, Result ...
2022年3月20日 · Auramine is a fluorochrome stain used to visualize acid-fast structures of various microorganisms especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , …
The Auramine Phenol stain is a variation of the acid-fast method developed by Robert Koch in 1882. Mycobacteria possess unique acid-fast characteristics that make the acid-fast staining techniques invaluable in detecting Mycobacteria species.
unique acid-fast characteristics that make the acid-fast staining techniques invaluable in detecting Mycobacteria species. Auramine phenol tends to show a higher sensitivity and specificity than the Kinyoun or ZN m
Auramine-Phenol Fluorochrome Staining: Introduction, Principle ...
Auramine-Phenol is a fluorochrome stain and used to visualize acid-fast structures of various microorganisms especially Mycobacterium tuberculosis and in modified form for Mycobacterium leprae, Nocardia species, Cryptosporidium parvum, Cyclospora cayetanensis , …