2021年5月3日 · We undertook a screen of mammalian gene regulatory factors to discover novel regulators of cardiac reprogramming in adult fibroblasts and identified the histone reader PHF7 as the most potent ...

2021年5月3日 · A new study now reports that the histone reader PHF7 enhances cardiac reprogramming via recruiting the chromatin remodelling SWI/SNF complex and key transcription factors to the cardiac super ...

2023年11月6日 · In this manuscript, we describe the ability of PHF7 to activate adult reprogramming in vitro in absence of canonical reprogramming factors, with PHF7, MEF2C, and TBX5 (PMT) or PHF7 and TBX5 (PT) alone. These minimalist PHF7 cocktails induced broad and unique cardiac transcriptomes in comparison to canonical cocktails.

2021年5月6日 · Now in Nature Cell Biology, Olson and colleagues report the identification of the histone reader PHF7 as a strong activator of the reprogramming of adult cardiac fibroblasts to induced...

2019年7月10日 · PHF7 deletion results in male infertility due to aberrant histone retention and impaired protamine replacement in elongated spermatids. Mechanistically, PHF7 can simultaneously bind histone H2A and H3; its PHD domain, a histone code reader, can specifically bind H3K4me3/me2, and its RING domain, a histone writer, can ubiquitylate H2A.

In this study, Lee et al. provide structural insight into the domain-specific functions that collaboratively contribute to the role of the RING-type E3 ligase PHF7 in histone ubiquitination. They further describe the molecular interactions that are ...

2023年10月13日 · Importantly, PHF7 is the first epigenetic factor shown to achieve efficient reprogramming in the absence of Gata4. Here, we highlight the underexplored necessity of cardiac epigenetic modifiers, such as PHF7, in harnessing chromatin remodeling and transcriptional complexes to overcome critical barriers to direct cardiac reprogramming.

We undertook a screen of mammalian gene regulatory factors to discover novel regulators of cardiac reprogramming in adult fibroblasts and identified the epigenetic reader PHF7 as the most potent activating factor. We validated the findings of this screen and found that PHF7 augmented reprogramming of adult fibroblasts ten-fold.

植物同源结构域 (PHD) 指蛋白 7 (PHF7) 是一种负责组蛋白泛素化的 RING 型 E3 泛素连接酶。 PHF7 包含三个锌指结构域：扩展 PHD (ePHD)、RING 结构域和 PHD。 虽然 RING 结构域的功能已被广泛了解，但其他两个结构域在 E3 连接酶活性中的作用仍然难以捉摸。 在这里，我们展示了 PHF7 与 E2 泛素结合酶 (E2) 复合物的晶体结构。 我们的结构表明，E2 在 RING 结构域和 C 端 PHD 之间被有效捕获，从而通过直接接触促进 E2 招募。 此外，通过体外结合和功能测定，我 …

2024年12月25日 · PHF7 (PHD Finger Protein 7) is a Protein Coding gene. Diseases associated with PHF7 include Bap1-Related Tumor Predisposition Syndrome and Bladder Squamous Cell Carcinoma. An important paralog of this gene is G2E3.