2022年8月10日 · above mentioned tests are used for confirmation of the Staphylococcus aureus. but first, you need to identify it by colony morphology then gram staining, microscopy, then catalase and oxidase test. when all of these test confirmed then apply above mentioned biochemical tests.

RapiDEC Staph is a test for presumptive identification of the principal human staphylococcal species, Staphylococcus aureus, S. epidermidis, and S. saprophyticus. The test includes control and test cupules for fluorogenic detection of coagulase and chromogenic substrates for alkaline phosphatase and beta-galactosidase.

All strains of Staphylococcus aureus and 83% of Staphylococcus epidermidis were phosphatase positive: all strains of Staphylococcus saprophyticus were negative. The method proved to be an accurate, reliable, economic and relatively rapid technique to detect phosphatase production.

2018年9月6日 · There are so many biochemical tests for Staphylococcus aureus, the well known causative agent of localized suppurative lesions. However, only a few reactions are most commonly used and are medically important for distinguishing pathogenic staphylococcus i.e. S. aureus from other non- pathogenic Staphylococci which are as follows:

The Staph-Zym method consists of 10 tests for β-glucosidase, β-galactosidase, β-glucuronidase, alkaline phosphatase, urease, pyrrolidonyl amonipeptidase, arginine dihydrolase, nitrate reduction, and acid from trehalose and maltose.

The test is described which uses p-nitrophenyl phosphate as the substrate and proved to be an accurate, reliable, economic and relatively rapid technique to detect phosphatase production. The ability of staphylococci to produce phosphatase is an important character in the identification of species in the genus.

2022年2月9日 · Biochemical Test of Staphylococcus aureus ­Basic Characteristics Properties (Staphylococcus aureus) Capsule Non-Capsulated Catalase Positive (+ve) Citrate

Phosphatase activity of Staphylococcus aureus: correlation of enzyme production with resistance to penicillin and phage pattern. J. Bacteriol. 86:1052–1056. 1963.—A simplified method for the determination of the phosphatase activity in different strains of staphylococci is described. The relationship between phosphatase production and ...

The API STAPH-IDENT strip utilizes a battery of 10 miniaturized biochemical tests, including alkaline phosphatase, urease, beta-glucosidase, beta-glucuronidase, and beta-galactosidase activity, aerobic acid formation from D-(+)-mannose, D-mannitol, D-(+)-trehalose, and salicin, and utilization of arginine.

1990年4月1日 · A Phosphatase-Novobiocin-Mannose-Inhibition Test (PNMI­Test) together with a high NaCI concentration (10%) in combination with a coagulase test seems to be an acceptable compromise for routine identification of the three most important staphylococcal urinary tract pathogens, S. aureus, S. epidermidis, and S. saprophyticus.