
Choosing a culture medium for SCNT and iSCNT reconstructed …
2017年11月10日 · In pigs, supplementation of in vitro maturation (IVM) culture with lanosterol improved preimplantation development of SCNT embryos, possibly by elevating lipid content …
In vitro and in vivo development of interspecies Asian elephant …
Interspecies somatic cell nuclear transfer (iSCNT) has an immense potential to rescue endangered animals and extinct species like mammoths. In this study, we successfully …
Nuclear-cytoplasmic incompatibility and inefficient development of pig …
We used pig-mouse cytoplasmic hybrids as a model to address nuclear-cytoplasmic incompatibility in iSCNT embryos. Fertilized murine zygotes were reconstructed by fusing with …
Transgenic Chicken, Mice, Cattle, and Pig Embryos by Somatic Cell ...
This study explored the possibility of producing transgenic cloned embryos by interspecies somatic cell nuclear transfer (iSCNT) of cattle, mice, and chicken donor cells into enucleated …
Transcriptomic insights into developmental arrest in fluorescent ...
As an endangered species with declining populations and limited oocyte availability, interspecies somatic cell nuclear transfer (iSCNT) combined with transcriptomic analysis holds promise for …
Development, embryonic genome activity and mitochondrial ...
Using a bovine-pig (inter-family) iSCNT model, we studied the basic characteristics of EGA: expression and activity of RNA polymerase II (RNA Pol II), formation of nucleoli (as an …
TSA和VPA处理对食蟹猴-猪异种体细胞核移植胚胎早期发育的影响
摘要: 食蟹猴-猪异种体细胞核移植(Interspecies somatic cell nuclear transfer, iSCNT)研究旨在由iSCNT胚胎建立具有与人类相似遗传背景的胚胎干细胞 (ESCs), 作为医学和基础科学研究的实 …
Development, embryonic genome activity and mitochondrial ... - rep
Using a bovine–pig (inter-family) iSCNT model, we studied the basic characteristics of EGA: expression and activity of RNA polymerase II (RNA Pol II), formation of nucleoli (as an …
Interspecies Somatic Cell Nuclear Transfer - ScienceDirect
2014年1月1日 · Domestic animals, such as cows, pigs, and rabbits – probably the best sources so far – have been used widely (Beyhan et al., 2007, Loi et al., 2011, Gomez et al., 2009). …
Assessing the effect of interspecies oocyte nucleolar material dosage ...
2020年10月1日 · As a proof-of-concept that the quantity of material, rather than its species of origin, can influence the iSCNT experiment outcomes, we tested whether porcine NLBs …
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