
Subcellular homeostasis of phytohormone auxin is mediated by …
2009年6月7日 · Here we show that Arabidopsis thaliana PIN5, an atypical member of the PIN gene family, encodes a functional auxin transporter that is required for auxin-mediated …
Mapping the membrane orientation of auxin homeostasis regulators PIN5 ...
2024年6月2日 · To assess the functionality of our newly generated constructs, we investigated root phenotype of the PIN5-GFP and PIN8-GFP fusions both in the WT and pin5-5 , or pin8-1 …
Differential Auxin-Transporting Activities of PIN-FORMED …
2010年5月3日 · In particular, immunogold-labeled PIN5-GFP was found in the ER around the nucleus (Mravec et al., 2009), suggesting that PIN5 might supply auxin to the nucleus so as to …
Functional Analysis of the Hydrophilic Loop in Intracellular ...
2014年4月1日 · Here, we observed the native PIN5 localization by expressing PIN5:GFP by its own promoter (∼2.5 kb from the start codon) in wild-type and pin5-3 mutant backgrounds, …
PIN5 is involved in regulating NH - Springer
2023年1月9日 · We show that high NH 4+ disturbs the subcellular IAA homeostasis by upregulating the expression of PIN5. Knockout of PIN5 resulted in elevated cytoplastic IAA …
Functional innovations of PIN auxin transporters mark crucial ...
All the GFP-fused canonical PIN proteins (SmPINR, PtPINH, and PtPING) from the vascular plants were polarly localized to the PM of the epidermal cells in Arabidopsis inflorescence …
PIN-Dependent Auxin Transport: Action, Regulation, and Evolution
However, a mutant version of PIN1, containing a green fluorescent protein (GFP) insertion that presumably interferes with a polarity-determining signal, localizes more apically (shootward) …
【读文献】【2013 Plant Cell 】PIN极化 - 简书
pin2-gfp定位在皮层细胞的顶端 在双突中,PIN1-GFP的荧光强度变弱,更弥散;双突中PIN2-GFP在侧边有时也有信号; 这表明,该基因的缺失,干扰了PIN1和PIN2的极化
Control of vein network topology by auxin transport - PMC
2015年11月11日 · Available evidence suggests that PIN5 transports auxin from the cytoplasm to the ER lumen [ 28, 33 ], and that PIN6 and PIN8 transport it from the ER lumen to the …
A novel putative auxin carrier family regulates intracellular auxin ...
2012年4月15日 · PIN1-type auxin carriers regulate the directional intercellular auxin transport at the plasma membrane. In contrast, atypical family member PIN5 regulates intracellular auxin …