提供 pJE202 载体/质粒图谱，全长序列，抗性，大小和元件信息。

Provide pJE202 vector/plasmid map, full length sequence, antibiotic resistance, size and other information

Find the DNA tube labelled “pJE202 plasmid” and, using your pipette, add 10uL to one tube of your competent cell mixture. Incubate this tube in the fridge (DO NOT FREEZE) for 30 minutes. Incubate the tube for 30 seconds in 42ºC (108ºF) water.

nant clones with pJE201 and pJE202 appeared to encode enzymatic functions necessary for light pro- duction in E. coli, as well as regulatory functions that

2016年8月12日 · Plasmid pJE202 also known as pVIB is an amp resistant plasmid that expresses the Lux operon genes from Vibrio fischeri. Originally from http://jb.asm.org/content/164/1/45.full.pdf. Plasmid comes already in E. coli DH10B strain of bacteria for immediate function. You can purify the plasmid from this strain if …

2019年5月9日 · This kit includes a DH10B based strain of E. coli that contains the Lux operon in the pJE202 plasmid. It also contains a 250mL glass bottle and enough media to grow up 8 cultures. (items pictured not included) How it works: Add one tube of the media to the glass bottle. Fill bottle with distilled water and shake until the media is dissolved.

To examine the genetic regulation of luminescence more closely, Escherichia coli catabolite repression mutants were transformed with a plasmid (pJE202) that contains V. fischeri genes specifying the luminescence enzymes and encoding regulatory functions for luminescence (the lux genes) or with plasmids (pJE413 and pJE455) containing ...

I recently bought a genetically modified e.coli (pje202/pVIB plasmid), which is bioluminescent. I don't want to grow the e.coli on antibiotic agar forever, because the antibiotic selection has ...

Episomal yeast two-hybrid vector for constitutive expression of LexA fused to the N-terminus of a protein. Also known as pLexA. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k …

Apparently, sigma 32 is not directly required for the transcription of luxICDABE in E. coli but is required for sufficient expression of groE, which is in turn required for the transcription of luxICDABE. This conclusion is supported by the finding that E. coli groE mutants containing pJE202 were weakly luminescent.