2015年12月17日 · This plasmid contains a hygromycin B resistance gene in the pKO7 43 vector backbone and the full length PaClpP gene under control of its wild-type promoter and terminator, with the codon for ...

2015年7月29日 · Deletion of PaRcf1 and PaRcf2 was performed with the method developed by El-Khoury and colleagues using the plasmid pKO7 30,44.

There is a problem with the plasmid I received. What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, …

2000年11月15日 · Here, we present a two-step technology which takes advantage of an Escherichia coli strain expressing the phage λ Red (gam, bet, exo) functions and involves (i) the construction in this strain of a recombinant cosmid by in vivo recombination between a cosmid carrying a genomic region of interest and a PCR-generated transformation marker flanked ...

2022年2月2日 · Here, we report how an oleic acid diet leads to longevity of the wild type and a PaAtg24 deletion mutant (ΔPaAtg24). The lifespan extension is linked to altered membrane trafficking, which abrogates the observed autophagy defects in ΔPaAtg24 by restoring vacuole size and the proper localization of SNARE protein PaSNC1.

A plasmid was subsequently isolated from an Amp r transformant and designated pKO7. The purA marker was isolated from pRM3100 by AatII digestion and inserted into the AatII site in the pKO7 backbone to form the deletion plasmid pKO9 (Amp r purA + ).

2022年3月21日 · generated according to a method developed by El-Khoury and colleagues using the plasmid pKO7 and 113. the strain ΔPaKu70 (El-Khoury et al., 2008; Kunstmann & Osiewacz, 2009). In short, approximately 1 114 kbp long fragments corresponding to the 5’ and 3’ flanking regions of the respective gene of interest

2022年1月15日 · Palm kernel oils (PKO1 – PKO7) of several different brands available in the Brazilian market were purchased. All oils were labeled as pure palm kernel oil by their respective manufacturers. Authentic palm kernel oil (PKO) was donated by Mejer Agroflorestal Company in Belém (Pará, Brazil).

2022年5月26日 · MICOS deletions strains were generated according to a method developed by El-Khoury et al. 45 using the plasmid pKO7 and the strain ΔPaKu70. 46 In short, approximately 1 kbp long fragments corresponding to the 5′ and 3′ flanking regions of the respective gene of interest were amplified by PCR using sequence-specific oligonucleotides ...

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