Eleven serotypes of AAV have thus far been identified, with the best characterized and most commonly used being AAV2. These serotypes differ in their tropism, or the types of cells they …

2015年3月30日 · Plasmids are important “vehicles” for the communication of genetic information between bacteria. The exchange of plasmids transmits pathogenically and environmentally …

Recombinant AAV gene therapy vectors (rAAV) can be made by cotransfection of 293 cells by two plasmids. The first plasmid contains the rAAV sequences and consists of the therapeutic …

Our AAV Rep-Cap plasmids work with our AAV Helper Free Systems to produce recombinant AAV more safely and easily, without the need for a helper virus. Choose one of these plasmids …

2020年12月3日 · You just need three plasmids to start making the AAVs you need for your experiment: and the transfer plasmid which contains the viral genome. In today’s blog post, …

2003年6月1日 · Cross-packaging of vector DNA derived from AAV-2, -3, or -6 was up to 10-fold more efficient using our novel plasmids, compared to a conservative adenovirus-dependent …

Today, plasmids are widely recognized not only as important factors facilitating genome restructuring but also as vehicles for the dissemination of beneficial characters within bacterial …

2024年9月13日 · DBP binds to single stranded DNA in a sequence independent manner and functions to increase the processivity of AAV DNA replication by stimulating elongation and …

1999年10月1日 · In this report we describe new helper plasmids (pSH3 and pSH5) that eliminate the Ad co-infection requirement. The helper plasmids express the AAV rep and cap genes and …

2007年1月1日 · Linear plasmids of eukaryotic microbes are contemporary manifestations of ancient viruses, which have adjusted to two cellular compartments during evolution, i.e., to the …