A plaque-forming unit (PFU) is a measure used in virology to describe the number of virus particles capable of forming plaques per unit volume. [1] It is a proxy measurement rather than a measurement of the absolute quantity of particles: viral particles that are defective or which fail to infect their target cell will not produce a plaque and ...

测定病毒 空斑形成单位 （PFU，plaque forming unit），用于流感病毒滴度测定及标定病毒的感染能力。 二、生物安全要求. 本实验采用 H1和H3亚型流感病毒 ，相关操作需要在 BSL-2级实验室 中进行。 三、实验材料

空斑形成单位（plaque forming unit），缩写pfu。 最初指在培养于琼脂培养基上的细菌被噬菌体杀死后形成空白斑，一个空斑为一个单位，后被拓展用于单层培养的动物细胞上形成一个空斑，一个空斑为一个单位。

After fixing and staining the infected cellular monolayer, plaques are counted in order to titer viral stock samples in terms of plaque forming units (pfu) per milliliter. A log drop should be noted between serial dilutions and, depending on plate size, between 5-100 plaques counted, with a negative control used as a reference.

MOI（Multiplicity of Infection）、PFU（Plaque-Forming Unit）、TCID50（50% Tissue Culture Infective Dose）和EID50（50% Egg Infective Dose）等都是病毒学实验中常用的参数。 MOI 是指病毒感染细胞的数量，通常表示为感染细胞中的病毒颗粒数与细胞数之比，是 病毒颗粒与细胞的接 …

Areas of cell destruction represent viral plaques and are enumerated as plaque forming units (PFU). Plaque formation is useful to identify a virus, determine the infectivity titer of a virus stock, or perform antiviral susceptibility testing.

2023年4月30日 · The number of plaques on a Petri dish are referred to as Plaque-Forming Units, or PFUs, and, providing the initial bacteriophage concentration was sufficiently dilute, should directly correspond to the number of infective phage particles in the original sample.

2025年3月24日 · By counting discrete plaques formed by viral replication and subsequent cell lysis, researchers determine the plaque-forming unit (PFU) concentration, a direct indicator of viral infectivity. This method is essential beyond basic viral quantification. Regulatory agencies such as the FDA and WHO require precise titration of live attenuated ...

2022年10月11日 · 空斑形成单位（plaque forming unit），缩写pfu。 最初指在培养于 琼脂 培养基上的细菌被噬菌体杀死后形成空白斑，一个空斑为一个单位，后被拓展用于单层培养的 动物细胞 上形成一个空斑，一个空斑为一个单位。

A plaque is an area of clearing in a confluent lawn of bacterial growth which represents the spot where a virus has landed, infected the bacteria it encountered, and lysed them. Each plaque represents a virus particle or plaque-forming unit (PFU).