Plasmid pMAL-c2X from Dr. Paul Riggs's lab is published in Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 10.1007/s00253-010-2696-y. Epub 2010 Jun 10. This plasmid is available through Addgene.

pMal-c2X载体价格800元，pMalc2X载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体抗性为氨苄，质粒大小为6646 bp，测序引物为MBP-F和pBad-R，主要用于大肠杆菌蛋白表达.

pMAL载体含有编码麦芽糖结合蛋白（Maltose Binding Protein, MBP）的大肠杆菌malE基因，其下游的多克隆位点便于目的基因插入，表达N端带有MBP的融合蛋白。 通过"tac"强启动子和malE翻译起始信号使克隆基因获得高效表达，并进一步利用MBP对麦芽糖的亲和性达到用Amylose柱 ...

提供 pMAL-c2x 载体/质粒图谱，全长序列，抗性，大小和元件信息。

融合表达麦芽糖结合蛋白MBP，蛋白定位于细胞周质。

Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present. Addgene has sequenced portions of this plasmid for verification. The results are shown below.

pMAL 载体含有编码麦芽糖结合蛋白 （Maltose Binding Protein, MBP）的大肠杆菌 malE 基因，其下游的多克隆位点便于目的基因 插入，表达 N 端带有 MBP 的融合蛋白。 通过"tac"强启动子和 malE 翻译起始信号使克隆基 因获得高效表达，并进一步利用 MBP 对麦芽糖的亲和性达到用 Amylose 柱对融合蛋白的一 步亲和纯化。

There is a problem with the plasmid I received. What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, and why do I have to order it? Welcome to Vector Database!

收到质粒后请先转化感受态(克隆菌株),再挑选单菌落重新提取后使用。 转化前请准确查找该质粒对应的抗生素、抗生素浓度、感受态(克隆菌株)和培养基温度。 如有必要请测序后使用。

2018年8月29日 · 3 rd Generation pMAL vectors: The third generation of pMAL vectors is distinguished by the addition of vectors that substitute an Enterokinase or Genenase I site for the Factor Xa site. These vectors are called pMAL-c2E and pMAL-p2E (Enterokinase), and pMAL-c2G and pMAL-p2G (Genenase).