Plasmid pMD2.G from Dr. Didier Trono's lab contains the insert VSV G. This plasmid is available through Addgene.

Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and expresses the VSV-G envelope protein under the control of the powerful mammalian CMV promoter.

Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.

Plasmid pMD2.G-VSV-G-mut from Dr. Howard Chang's lab contains the insert VSV-G and is published in Cell. 2022 Dec 22;185(26):4904-4920.e22. doi: 10.1016/j.cell.2022.11.016. Epub 2022 Dec 13. This plasmid is available through Addgene.

Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.

pMD2.G: 0.72 pmol: Transfer Plasmid* 1.64 pmol: OptiPro SFM to total volume: 500 μL *Plasmid concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid DNA purification should include an endotoxin removal step.

pMD2.G Notes Addgene diagnostic digest- NotI, HindIII This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: NotI, HindIII and NotI+HindIII double digest.

ID Plasmid Purpose; 12259: pMD2.G: VSV-G envelope expressing plasmid: 12260: psPAX2: 2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

Plasmid pMD2.G-H8A/K47Q from Dr. Feng Zhang's lab contains the insert VSV-G and is published in Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8. This plasmid is available through Addgene.

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).