pmirGLO Vector for quantitatively analyzing microRNA (miRNA) activity by inserting miRNA binding sites downstream of a firefly luciferase gene. Sequence Author : Promega

pmirGLO Dual-Luciferase miRNA Target Expression Vector (E1330) 是为miRNA靶标研究而设计的载体，可通过将 miRNA 的靶序列插入到主报告基因萤火虫萤光素酶基因（luc2）的下游或者 3' 端而实现。 萤火虫萤光素酶表达的下降意味着内源性的或外源引入的 miRNA 与克隆的 miRNA 的靶序列发生了结合。 这一载体是基于 Promega 的双报告基因技术，萤火虫萤光素酶基因（luc2）作为主报告基因，用于监控 miRNA 的调节，海肾萤光素酶基因（hRluc-neo）作为内 …

The pmirGLO Dual-Luciferase miRNA Target Expression Vector (a–d) is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites 3´ of the firefly luciferase gene (luc2 ).

pMir-Glo载体价格1500元，pMirGlo载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Ampicillin，质粒大小为7350bp，是荧光素酶报告系统的pMirGlo载体。

pmirGLO 质粒 pmirGLO Plasmids 使用手册V1.0 北京天恩泽基因科技有限公司 北京市海淀区上地信息路12号北京市留学人员海淀创业园中关村创业大厦D405 网址：www.tiandz.com；电话：400-6765278；电邮：[email protected]

The pmirGLO Dual-Luciferase miRNA Target Expression Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites 3′ of the firefly luciferase gene (luc2). These target sites can be introduced by cloning putative miRNA binding sites alone, or the 3′ untranslated region (UTR) of a gene of ...

Description: The pmirGLO Dual-Luciferase miRNA Target Expression Vector(a,b) is designed to quantitatively evaluate microRNA (miRNA) activity by inserting miRNA target sites 3 of the firefly luciferase gene (luc2).

pmirGLO载体是海肾荧光素基因（hRluc）和萤火虫荧光素基因（luciferase）在同一个载体同时表达，用SV40 promoter启动hRluc荧光和NeoR抗性表达，PGK promoter启动luciferase荧光表达。

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA ...

The pmirGLO Vector was designed to quantitatively assess miRNA activity, which is achieved by inserting the target sequence of the miRNA downstream or at the 3' end of the firefly luciferase gene (luc2).