2016年3月31日 · Terminators are found downstream of the gene to be transcribed, and typically occur directly after any 3’ regulatory elements, such as the polyadenylation or poly(A) signal. …

2024年11月14日 · For example, inserting a polyadenylation signal downstream of the transcription start site will terminate transcription of most of the ncRNA (Figure 1D). While this …

2020年8月11日 · The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes RNA transcript longevity. The polyA is located at the end of the AAV …

2016年3月17日 · The polymerase will elongate the mRNA until it reaches the gene end signal, where it will terminate transcription and polyadenylate the mRNA. These mRNAs are …

2024年5月23日 · A cis-splicing vector served as positive control (cis-ctrl). v1, vector 1; v2, vector 2; BD, binding domain; SDS, splice donor site; SAS, splice acceptor site; pA, polyadenylation …

2023年10月19日 · Figure 1: Recombinant AAV vector with ITRs that flank the gene cassette, which contains a promoter, the gene of interest, and a polyA signal. Created with …

2015年12月23日 · In the case of pHlash, the signal from the fluorophore is pH-dependent. Signal is simultaneously acquired from the the fluorescent and luminescent siganls and a ratio is …

MXS-chaining was designed to create plasmids for fluorescence imaging or flow cytometry applications in mammalian cells. The modules used in this method include fluorescent …

2014年3月25日 · Although plasmids do not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian cells.

2024年11月21日 · Plus, the luminescence wavelength (460 nm) is shorter than many popular fluorescence markers like GFP (510 nm), allowing co-imaging. And because bioluminescent …