Polysomes can be size-fractionated by sucrose density gradient centrifugation. The more specific mRNA in polysomes implies more active translational status of the mRNA. Prepare neuroblastoma cell line SKN-SH cells 2.5-5 × 10 7 /group, normally 2 to 3 150 cm 2 flasks with 50 ml of RPMI-1640 medium.

Polysome is a strategy employed in bacteria and eukaryotes to accelerate the overall rate of protein biosynthesis. Bacterial mRNA lacks intron elements and does not require further processing once it is transcribed.

A polysome, also known as a polyribosome, is a complex formed by multiple ribosomes simultaneously translating a single mRNA strand. This structure allows for the efficient synthesis of many copies of a protein from one mRNA molecule.

A polysome (or a polyribosome) is a group of two or more ribosomes translating an mRNA sequence simultaneously. The polysomes will appear as beads on a string (each 'bead' represents a ribosome ; the ‘string’ is the mRNA strand) In prokaryotes, the polysomes may form while the mRNA is still being transcribed from the DNA template

Polysome Profiling Without Gradient Makers or Fractionation Systems. Polysome fractionation by sucrose density gradient centrifugation is a powerful tool that can be used to create ribosome profiles, identify specific mRNAs being translated by ribosomes, and analyze polysome associated factors.

A polysome, also known as a polyribosome, is a complex structure formed when multiple ribosomes bind to a single mRNA molecule. These ribosomes move along the mRNA like beads on a string, each translating a separate copy of the protein encoded by the mRNA.

A rosette or helical cluster of ribosomes is brought together by a strand of messenger RNA to form a polysome (mRNA). They include a piece of the genetic code that is translated by each ribosome and are employed in the synthesis of numerous copies of the same polypeptide.

Polysome is a group of ribosomes involved in mRNA translation. A polyribosome (polysome or ergasome) is bound to an mRNA molecule like “beads” on a “thread”. So, polysomes consist of a complex of an mRNA molecule and two or more ribosomes …

polysome: Group of ribosomes functionally related and held together by a strand, presumable mRNA.

Polysomes are stable protein–ribosome–mRNA complexes, which can be used to replace live bacteriophages as the display vehicle for recombinant antibody fragments [74•,75]. The polysomes are formed by preventing release of newly synthesised and correctly folded protein from the ribosome.