植物叶绿体型PPDK具有一段转运肽，而细菌、原生动物PPDK，以及植物中细胞质型PPDK不具有此结构，此外，它们的一级结构相似性较高。 玉米PPDK基因含有19个外显子，约12kb。 在其5’侧翼序列区存在一个典型的TATA元件，一个颠倒的CCAAT样序列，以及位于—51到—42的GTCGCGCCC序列（在动物细胞中此富含GC的序列为SP1蛋白的结合位点）。 Clackin等人发现玉米中的PPDK基因具有两个不同的转录起始位点，分别受两个启动子控制。 其中，长转录本 …

Phospho-PDK1 (Ser241) Antibody detects PDK1 only when phosphorylated at Ser241. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser241 of human PDK1. Antibodies are purified by protein A and peptide affinity chromatography.

2020年5月11日 · Here, we demonstrate that 3′-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE1 (PDK1), which is conserved in plants and mammals, functions as a molecular hub that perceives...

2020年5月11日 · Our data indicate that PDK1 regulates polar auxin transport by activating AGC1 clade kinases, resulting in PIN phosphorylation. PDK1 is a conserved protein serine/threonine kinase in eukaryotes.

2022年4月6日 · 3-phosphoinositide-dependent kinase 1 (PDK1) is an essential serine/threonine protein kinase, which plays a crucial role in cell growth and proliferation. It is...

2012年6月15日 · 3-Phosphoinositide-dependent protein kinase-1 (PDK1) is a 63-kDa Ser/Thr protein kinase that phosphorylates and regulates several downstream signaling pathways including several "AGC" family kinases such as Akt/protein kinase B, protein kinase C (PKC), p70S6K, and serum and glucocorticoid-regulated kinase (SGK) [ 14, 15 ].

Consistent with previous reports that O2 regulates PPDK1 and PPDK2 (31, 32), coexpression of Pppdk1-LUC or Pppdk2-LUC with 35S-O2 resulted in a 17-or 44-fold increase in LUC activity,...

2016年4月18日 · 玉米 ppdk 基因包括2个成员（ppdk1和ppdk2），二者在根、叶 片和种子中均表达[8－9]。在 叶片中 ppdk1 基因主要 参与光 合 作 用，在种 子 中 调控淀粉的生 物 合 成代

2001年2月1日 · We have cloned and characterised a gene that encodes a putative pyruvate phosphate dikinase (PPDK) from Trypanosoma cruzi, an enzyme that catalyses the reversible conversion of phosphoenolpyruvate to pyruvate. PPDK is absent in mammalian cells, but has been found in a wide variety of other organisms, including plants and bacteria.

【摘 要】 [目的]分析玉米胚乳可溶性淀粉合成酶 (SSⅠ)与质体型糖酵解途径关键催化酶丙酮酸磷酸双激酶 (PPDK1)的蛋白互作关系,揭示可能发生互作的亚细胞位置. [方法]采用酶切连接的方法构建326-CYCHA-ss1和326-CYNEE-ppdk1双分子荧光互补表达载体,转化农杆菌EHA105,瞬时浸染烟草叶片组织,激光共聚焦显微镜下观察SSⅠ和PPDK1相互作用产生的荧光信号. [结果]双酶切试验鉴定表明,326-CYCHA-ss1和326-CYNEE-ppdk1重组载体构建正确；PCR结果证实,植物表达载体 …